摘要
采用碱提酸沉方法从双液相棉粕中制取棉籽蛋白,pH值对蛋白质和植酸的萃取率及沉淀率影响很大.当pH11～11.5时,棉籽蛋白的萃取率高达73.5%,此时植酸的萃取率较低（约22%）;当pH5.0时棉籽蛋白的沉淀率（76.8%）和植酸的萃取率（88.7%）同时达到最大值.通过正交试验,考察了pH值、温度、絮凝时间等因素对棉籽蛋白沉淀率的影响,结果表明：温度越低沉淀率越高,絮凝时间延长有利于棉籽蛋白的沉淀.适宜的蛋白质沉淀条件为：pH4.8,温度5℃,絮凝时间10min,棉粕蛋白的沉淀率为77.5%.
The influence of pH on extraction and precipitation characters of cottonseed protein and phytic acid were measured in order to reduce the phytic acid content in the protein isolates. In the range of pH 11 - 11.5 the protein extraction rate is high （73.52%）, but the extraction rate of phytic acid is minor （22%）. The precipitation rate of protein and extraction rate of phytic acid both reach maximum （76.8% and 88.7% respectively） at pH 5. 0. Based on these experimental results the content of phytic acid in cottonseed protein isolates can be removed as low as possible. The influences of pH, temperature, and flocculation time on the protein precipitation were investigated by an orthogonal experiment. Relatively low temperature and long flocculation time are beneficial to the protein precipitation. The obtained proper conditions for the protein precipitation are as follows： pH, 5. 0, temperature, 5℃ ; and flocculation time 〉 10min. A cottonseed meal protein precipitation rate of 77.5% was obtained under these conditions.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2006年第2期65-68,共4页
Journal of the Chinese Cereals and Oils Association
关键词
棉籽蛋白
植酸
萃取率
沉淀率
cottonseed protein,phytic acid,extraction rate,precipitation rate
