摘要
以“双低”菜籽脱皮脱脂粕为原料,用碱溶酸沉法制备菜籽分离蛋白,通过对各种因素的分析得到制备菜籽分离蛋白的最佳工艺条件为:在pH为11的条件下,用NaOH溶液浸提4次,每次浸提35 min,固液比为1:12,浸提温度为50℃。在最佳条件下,产品蛋白质含量达86.12%,产品得率为24.35%。在碱溶酸沉前,用pH为5的8倍水脱植酸,用85%的乙醇、固液比1:4脱单宁,可制得低植酸、低单宁的“双低”菜籽分离蛋白。
Rapeseed protein was prepared by alkali extraction and acid precipitation from double-low defatted ropeseed meal. The optimum conditions were established, which included alkaline of pH （ pH = 11.0）, extracting temperature （50℃ ）, extracting times（4）, extracting time （35 min）and the ratio of rapeseed meal to solvent（1：12）. Under the best conditions, the protein content of product is 86.12% and the yield is 24.35%. After phytic acid was removed by 8 times of water with pH 5.0 and tannin was removed by 4 times of 85% ethanol, double-low rapeseed protein isolate with low-phytic acid and low-tannin was prepared by alkali extraction and acid precipitation.
出处
《中国油脂》
CAS
CSCD
北大核心
2005年第8期38-41,共4页
China Oils and Fats
关键词
“双低”菜籽脱脂粕
菜籽分离蛋白
植酸
单宁
硫甙
double-low defatted rapeseed meal
rapeseed protein isolate
phytic acid
tannin
glucosinolate
