摘要
目的:建立小儿清咽颗粒中牛蒡苷的含量测定高效液相色谱法。方法:Shim-Pack C18柱,流动相为乙腈-水(26:74), 检测波长277 nm,流速0．8 ml·min-1。结果:线性范围为1．0-3．5μg。平均加样回收率为98．9％,RSD为0．9％。结论:方法简便快速,重现性良好,能有效控制小儿清咽颗粒的质量。
Objective： To establish a method for the determination of arctiin in Xiaoer Qingyan Granules by HPLC. Method： The Shim-Pack C18 column（200 mm×4.6 mm,5μm） was used,the mobile phase was Acetonitrile-water（26： 74） ,the detection wavelength was at 277 rim, the flow rate was 0.8 ml·min^-1. Result： The arctiin was separated well, the linear range of Arctiin was 1.0-3.5μg. The average recovery was 98.9% ,RSD was 0.9%. Conclusion： The method is simple and rapid with good reproducibility,and can be used for quality control of Xiaoer Qingyan Granule.
出处
《中国药师》
CAS
2006年第1期44-45,共2页
China Pharmacist
