摘要
目的建立HPLC法同时测定小儿清咽颗粒中单咖啡酰酒石酸、连翘酯苷A、菊苣酸、连翘苷、牛蒡苷、哈巴俄苷的含量。方法该药物70%甲醇提取液的分析采用Agilent Zorbax SB-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长278、330 nm。结果6种成分在各自范围内线性关系良好(r≥0.9994),平均加样回收率96.80%~102.61%,RSD 0.26%~1.93%。结论该方法简便准确,可用于小儿清咽颗粒的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of monocaffeyltartaric acid,forsythiaside A,chicoric acid,phillyrin,arctiin and harpagoside in Waigan Fenghan Granules.METHODS The analysis of 70%methanol extract of this drug was performed on a 30℃thermostatic Agilent Zorbax SB-C_(18)column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 278,330 nm.RESULTS Six constituents showed good linear relationships within their own ranges(r≥0.9994),whose average recoveries were 96.80%-102.61%with the RSDs of 0.26%-1.93%.CONCLUSION This simple and accurate method can be used for the quality control of Xiao’er Qingyan Granules.
作者
何艳
胡小祥
刘洋伽
戴瑞
唐佳齐
张辉
HE Yan;HU Xiao-xiang;LIU Yang-jia;DAI Rui;TANG Jia-qi;ZHANG Hui(College of Pharmacy,Xiangnan University,Chenzhou 423000,China;Chenzhou Municipal Center for Food and Drug Control,Chenzhou 423000,China)
出处
《中成药》
CAS
CSCD
北大核心
2023年第12期3906-3909,共4页
Chinese Traditional Patent Medicine
基金
湖南省自然科学基金科药联合项目(2021JJ80023,2021JJ80074)
郴州市科技计划项目重点研发项目(zdnk202101)
湘南学院横向项目(合同号2022E88)。
关键词
小儿清咽颗粒
化学成分
HPLC
Xiao’er Qingyan Granules
chemical constituents
HPLC