摘要
目的建立同时测定中风合剂中梓醇、哈巴苷、绿原酸和芍药苷4种有效成分含量的高效液相色谱多波长法。方法色谱柱为Welch XB-C18柱(250 mm×4.6 mm,5μm),流动相A为0.06%磷酸溶液、B为乙腈(梯度洗脱),流速为1.0mL/min,检测波长分别为200,231,327 nm,柱温为30℃,进样量为10μL。结果梓醇、哈巴苷、绿原酸、芍药苷进样量分别在0.156~1.248μg,0.154~1.232μg,0.136~1.086μg,0.231~1.845μg范围内与峰面积线性关系良好(r=0.999 9,0.999 9,1.000 0,0.999 9),平均加样回收率分别为99.47%,99.54%,99.89%,99.94%,RSD分别为0.54%,0.91%,0.23%,0.35%(n=6)。结论该方法操作简单、专属性强,可用于中风合剂的质量控制。
Objective To establish a multiwavelength HPLC method for simultaneous determination of 4 active components of catalpol,harpagide,chlorogenic acid and paeoniflorin in Zhongfeng Mixture.Methods The chromatographic column was Welch XB-C18 column(250 mm×4.6 mm,5μm),the mobile phase A was 0.06%phosphoric acid solution,the mobile phase B was acetonitrile(gradient elution),the flow rate was 1.0 m L/min,the detection wavelength was 200,231,327 nm,the column temperature was 30℃and the sample size was 10μL.Results The linear range was 0.156-1.248μg(r=0.999 9)for catalpol,0.154-1.232μg(r=0.999 9)for harpagide,0.136-1.086μg(r=1.000 0)for chlorogenic acid and 0.231-1.845μg(r=0.999 9)for paeoniflorin.The average recoveries were 99.47%,99.54%,99.89%,99.94%,RSDs were 0.54%,0.91%,0.23%and 0.35%(n=6).Conclusion The method is simple and specific,which can be used for the quality control of Zhongfeng Mixture.
作者
江建丽
JIANG Jianli(Fujian Manping of Food and Drug Inspection and Testing Center,Manping,Fujian,China 353000)
出处
《中国药业》
CAS
2019年第15期23-25,共3页
China Pharmaceuticals
关键词
高效液相色谱多波长法
中风合剂
梓醇
哈巴苷
绿原酸
芍药苷
含量测定
multiwavelength HPLC
Zhongfeng Mixture
catalpol
harpagide
chlorogenic acid
paeoniflorin
content determination
