摘要
目的：建立同时测定艾滋病口腔含漱液中梓醇、哈巴苷和哈巴俄苷含量的方法。方法：采用双波长超高效液相色谱法,色谱柱为Eclipse Plus C18,流动相为乙腈-0.05%磷酸溶液（梯度洗脱）,流速为0.2 ml/min,波长切换,0~8 min在210 nm波长测定梓醇和哈巴苷的含量,8~15 min在280 nm波长测定哈巴俄苷的含量,柱温为30℃,进样量为2μl。结果：梓醇、哈巴苷、哈巴俄苷进样量分别在0.018 5~0.129 2、0.014 0~0.098 3、0.015 7~0.110 0μg范围内与各自峰面积呈良好的线性关系（r=0.999 6、0.999 7、0.999 7）;精密度、稳定性、重复性试验的RSD≤0.77%;平均加样回收率分别为99.44%、99.90%、100.20%,RSD分别为0.75%、0.82%、0.73%（n=6）。结论：该方法操作简便、重复性好,可以更有效地控制艾滋病口腔含漱液的质量。
OBJECTIVE： To establish a method for simultaneous determination of the content of catalpol, harpagide and hapagoside in AIDS oral gargle. METHODS： Dual-wavelength UPLC was performed on the column of Eclipse Plus C18 with mobile phase of acetonitrile-0.05% phosphoric acid （gradient elution） at the flow rate of 0.2 ml/min. Wavelength was switching, it was set at 210 nm to determine the contents of catalpol and harpagide in the first 8 min and then changed to 280 nm to determine the content of hapagoside in 8-15 min. The column temperature was 30 ℃ ,and injection volume was 2 μl. RESULTS： The linear range was 0.018 5-0.129 2 μg （r=0.999 6） for catalpol, 0.014 0-0.098 3 μg （r=0.999 7） for harpagide and 0.015 7-0.110 0 μg（r= 0.999 7） for hapagoside; RSDs of precision, stability and reproducibility tests were no more than 0.77%; average recoveries were respectively 99.44% （RSD=0.75%, n=6）, 99.90% （RSD=0.82%, n=6） and 100.20% （RSD=0.73%, n=6）. CONCLUSIONS ： The method is simple can reproducible, and can be used for effectively quality control.
出处
《中国药房》
CAS
北大核心
2015年第24期3414-3416,共3页
China Pharmacy
基金
广西壮族自治区卫生厅中医药科技专项课题(No.GZYZ1121)
