摘要
目的建立一测多评法同时测定百合固金片中哈巴苷、哈巴俄苷、梓醇、毛蕊花糖苷、焦地黄苯乙醇苷B1、氧化芍药苷、芍药内酯苷、芍药苷、苯甲酰芍药苷等9种成分含量。方法采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm);乙腈(A)-0.2%磷酸溶液(B)为流动相,梯度洗脱;检测波长分别为210 nm(哈巴苷、哈巴俄苷、梓醇),330 nm(毛蕊花糖苷、焦地黄苯乙醇苷B1)和230 nm(氧化芍药苷、芍药内酯苷、芍药苷、苯甲酰芍药苷);流量0.9 mL·min^-1;柱温25℃;以芍药苷为参照,建立哈巴苷、哈巴俄苷、梓醇、毛蕊花糖苷、焦地黄苯乙醇苷B1、氧化芍药苷、芍药内酯苷和苯甲酰芍药苷与芍药苷的相对校正因子,计算其他8种成分的含量,实现一测多评,同时与外标法测定结果进行对比,验证一测多评法的准确性和科学性。结果一测多评法所得结果与外标法无显著差异。结论一测多评的质量评价模式用于百合固金片的质量控制可行,结果准确。
Objective To establish a quantitative analysis of multi-components by single-marker(QAMS) method for simultaneous determination of nine components(harpagide,harpagoside,catalpol,verbascoside,jionoside B1,oxypaeoniflorin,albiflorin,paeoniflorin and benzoylpaeoniflorin) in Baihe Gujin Tablet. Methods A Diamonsil C18column(250 mm×4.6 mm,5 μm) was used.The mobile phase was acetonitrile(A)-0.2% phosphate acid solution(B) at the flow rate of 0.9 mL·min^-1 for gradient elution.The determination wavelengths were 210 nm for harpagide,harpagoside and catalpol,330 nm for verbascoside and jionoside B1,230 nm for oxypaeoniflorin,albiflorin,paeoniflorin and benzoylpaeoniflorin.The column temperature was 25 ℃.Paeoniflorin was used as the internal reference substance.The relative correction factors(RCF) between the paeoniflorin and harpagide,harpagoside,catalpol,verbascoside,jionoside B1,oxypaeoniflorin,albiflorin,benzoylpaeoniflorin were established to detect the quantitation of paeoniflorin and calculate the quantitation of the other eight constituents.The external standard method was used for quantitating the nine constituents,and the method was evaluated by comparing to the quantitative results between external standard method and QAMS method. Results The results obtained by QAMS approximated those obtained by external standard method. Conclusion The use of relative correction factor is feasible to assay the content of nine components in Baihe Gujin Tablet,The QAMS method can be used for the quality assessment of Baihe Gujin Tablet.
作者
陈海燕
刘华东
李冰
覃佩莉
CHEN Haiyan;LIU Huadong;LI Bing;QIN Peili(Guangxi Medical College,Nanning 530023,China;Guangxi Baiyunshan Yingkang Pharmaceutical LTD.,Nanning 530023,China)
出处
《医药导报》
CAS
北大核心
2020年第3期370-375,共6页
Herald of Medicine
关键词
百合固金片
一测多评法
校正因子
相对
色谱法
高效液相
Baihe Gujin tablet
Quantitative analysi of multi-components by single-marker
Correlation factor,relative
Chromatography,high performance liquid
