摘要
目的:建立UPLC法同时测定竹节参中7种皂苷含量,并结合化学计量学分析评价其质量。方法:采用ACQUITY UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),流动相为水(A)—乙腈(B),梯度淋洗;流速0.4 mL/min,温度30℃,进样体积1μL,检测波长203 nm。应用化学计量学中聚类分析、主成分分析及质量波动分析对含量测定结果进行识别,以分析不同产地竹节参药材间相似性及差异性。结果:7种皂苷(人参皂苷Rg_(1)、人参皂苷Re、人参皂苷Rb_(1)、竹节参皂苷Ⅴ、竹节参皂苷Ⅳ、竹节参皂苷Ⅳa与假人参皂苷RT_(1))在核定范围内线性关系良好(R^(2)≥0.9994);平均加样回收率在99.00%~104.37%;人参皂苷Rg_(1)、人参皂苷Re、人参皂苷Rb_(1)、竹节参皂苷Ⅴ、竹节参皂苷Ⅳ、竹节参皂苷Ⅳa与假人参皂苷RT_(1)的含量范围分别为0.21~18.85,0.59~2.82,1.25~8.12,59.14~97.16,22.21~47.19,15.97~32.66,0.07~34.09 mg/g。通过聚类分析和主成分分析可将9批竹节参样品分为3类,竹节参样品S6为Ⅰ类,S3、S7和S8为Ⅱ类,S1、S2、S4、S5和S9为Ⅲ类;通过质量波动分析发现人参皂苷Rg_(1)与假人参皂苷RT_(1)含量波动大。结论:竹节参中7种皂苷成分含量同时测定的UPLC法及化学计量学综合评价可用于竹节参的质量评价。
Objective:This study aimed to establish UPLC method for simultaneous determination of seven kinds of saponins in Panax japonicus and evaluate its quality combined with chemometrics analysis.Methods:ACQUITY UPLC BEH C_(18) column(2.1 mm×100 mm,1.7μm)was used,and the mobile phase was water(A)-acetonitrile(B)with gradient elution.The flow rate was 0.4 mL/min,at 30℃,and the injection volume was 1μL detected 203 nm.Cluster analysis,principal component analysis(PCA)and quality fluctuation analysis in chemometrics were used to identify the content determination results,in order to analyze the similarities and differences between Panax japonicus in different regions.Results:Seven saponins(ginsenoside Rg_(1),ginsenoside Re,ginsenoside Rb_(1),chikusetsusaponin Ⅴ,chikusetsusaponin Ⅳ,chikusetsusaponin Ⅳ a and pseudoginsenoside RT_(1))had good linear relationship within the approved range(R^(2)≥0.9994).The average recovery rate was 99.00%~104.37%.The contents of ginsenoside Rg_(1),Re,Rb_(1),chikusetsusaponin Ⅴ,Ⅳ,Ⅳa and pseudoginsenoside RT_(1) were 0.21~18.85,0.59~2.82,1.25~8.12,59.14~97.16,22.21~47.19,15.97~32.66 and 0.07~34.09 mg/g,respectively.According to the results of cluster analysis and principal component analysis,nine batches of P.japonicus samples were divided into three categories:S6 was classified as Ⅰ;S3,S7 and S8 were classified as Ⅱ,and S1,S2,S4,S5;S9 were classified as Ⅲ.The quality fluctuation analysis showed that the contents of ginsenoside Rg_(1) and pseudoginsenoside RT_(1) fluctuated greatly.Conclusion:Simultaneous determination of seven saponins in P.japonicus by UPLC combimed with chemometrics comprehensive evaluation could be used for quality evaluation of P.japonicus.
作者
张思敏
张也
伍红年
刘隆基
申佩瑶
严建业
杨磊
ZHANG Si-min;ZHANG Ye;WU Hong-nian;LIU Long-ji;SHEN Pei-yao;YAN Jian-ye;YANG Lei(Institute of Innovation and Applied Research in Chinese Medicine,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;The First Hospital Affiliated to Hunan University of Chinese Medicine,Changsha,Hunan 410007,China;Department of Pharmacy,People's Hospital of Liuyang,Changsha,Hunan 410399,China;College of Life Science and Technology,Central South University of Forestry and Technology,Changsha,Hunan 410004,China)
出处
《食品与机械》
北大核心
2022年第3期65-70,79,共7页
Food and Machinery
基金
湖南省自然科学基金项目(编号:2020JJ4470)
湖南省教育厅资助科研项目(编号:21A0169)
湖南省中医药科研计划课题项目(编号:202072)。
关键词
竹节参
产地
UPLC
皂苷
聚类分析
主成分分析
Panax japonicus C.A.Mey
regions
UPLC
saponins
cluster analysis
principal component analysis
