摘要
以休眠期花魔芋的球茎、根壮茎、鳞片、花芽、叶芽为外植体诱导再生植株,结果表明:接种于MS+6- BA1mg／L+NAA0．5mg／L的培养基中的花芽内层粉红色鳞片为最佳外植体,培养期间不发生溶胀,污染率、褐变 率、死亡率低,愈伤率达100％,继续培养可分化芽、根,于3月底～4月中旬形成完整植株．能在魔芋播种季节进行 试管苗移栽,保证了种芋的生长时间,有利于提高产量和生产效益．
A · Knojac induce callus by using its corms, squamas, sproutings and rhizomes as the explants, the chosen medium is MS ＋ 6 - BA 1 mg/L ＋ NAA 0.5 mg/L, it can be found that the squamas of the flower buds in the middle layer and the leaf buds are the optimum medium to be explanted . Highest rate 100% of inducement of callus could be developed into plants when it was obtained on MS ＋ 6 - BAlmg/L ＋ NAA 0.5 mg/L. It is efficient to inducing callus under 6 - BA is 1-2 mg/L and NAA is 0.5 - 1 rag/L, KT is inefficient to inducing.
出处
《湖北民族学院学报（自然科学版）》
CAS
2005年第4期375-377,共3页
Journal of Hubei Minzu University(Natural Science Edition)
基金
国家科技攻关计划项目资助(2003BA90A05)湖北省教育厅科研项目(2004D001).
关键词
休眠期
花魔芋
植株再生
A · Knojac
callus
tissues culture
