摘要
以魔芋茎尖、幼芽为外植本，接种于１／２ＭＳ＋１．０ｍｇ／ＬＢＡ＋０．０１ｍｇ／ＬＮＡＡ的培养基中，茎尖、幼芽逐渐生长直接形成幼芽或幼苗；或从茎尖、幼芽由来的膨大的块茎组织表面诱导出幼芽。膨大的块茎组织分割后接种于ＭＳ＋０．０１ｍｇ／Ｌ＋０．０１ｍｇ／ＬＮＡＡ的培养基中进行增殖培养，同时从增殖的块茎组织表面不断地诱导出幼芽。幼芽切块转入不含激素的ＭＳ培养基中，形成幼苗。幼苗切块转入ＭＳ＋０．１ｍｇ／ＬＮＡＡ的生根培养茎中，幼苗生根，形成完整的植株。试管苗移栽６个月后获得干块茎。
Shoot tips and buds in Amorphophallus Konjac cultured on 1/2 Murashige and Skoog hasal salts medium supplemented with 1. 0 mg/L BA and 0. 01 mg/L NAA. and buds from shoot tips was observed. Shoot tips explant and buds explant was proliferated and proliferated tissue were produced new thes. Proliferated tissue were transferred to MS medium supplemeted with 1. 0mg/L BA and 0. 01mp/L NAA and those were produed new buds. New buds could grow into rootted seedling on MS muelicm supplemented not exogenous hormones. When the rootless seelings transfered on MS medium supplemented with 0. 1 mg/L NAA. Plantlets with roots would be gotten. The micro-tubers were produced from Plantlets on cultivation under glass aftter 6 months.
出处
《生物技术》
CAS
CSCD
1994年第1期19-21,共3页
Biotechnology
关键词
魔芋
茎尖
组织培养
植株再生
Amorphophallus Konjac
Shoot tips
Tissue Culture
Plant Regeneration
