摘要
[目的]通过器官发生直接途径再生植株的方式,研究建立完善的“花魔芋”组织培养快繁技术体系。[方法]以“花魔芋”球茎的顶芽为外植体,研究不同的灭菌方法、培养基、继代周期等因素对“花魔芋”组织培养的影响。[结果]适合“花魔芋”顶芽灭菌的方法是0.20%HgCl 2灭菌10~15 min;适合初代培养的培养基是MS+6-BA 1.0~3.0 mg/L+NAA 0.2~0.5 mg/L;适合增殖培养的培养基是MS+6-BA 1.0~2.0 mg/L+NAA 0.1~0.2 mg/L;适合生根培养的培养基是1/2MS+NAA(或IBA)0.1~0.2 mg/L+活性炭0.1%。[结论]得出了一套完善的“花魔芋”组织培养快繁技术体系,能育出有较高素质的“花魔芋”种苗,可以推广应用于“花魔芋”的工厂化育苗,同时也为其他品种的魔芋组织培养快繁技术研究提供参考。
[Objective]To study and further establish a perfect technology system of rapid propagation in tissue culture of Amorphophallus konjac,by means of direct organogenesis and plant regeneration.[Method]Apical buds sprouting from the corms of Amorphophallus konjac were used as explants,and effects of different sterilization method,medium and subculture cycle on tissue culture of Amorphophallus konjac were studied.[Result]The results showed that the suitable explants sterilization method was treatment 10-15 min by 0.20%HgCl 2,suitable primary culture medium was MS+6-BA1.0-3.0mg/L+NAA0.2-0.5mg/L,suitable proliferation medium was MS+6-BA 1.0-2.0 mg/L+NAA 0.1-0.2 mg/L,and suitable rooting medium was 1/2MS+NAA(or IBA)0.1-0.2mg/L+activated carbon 0.1%.[Conclusion]The technology system was acquired in this study,which can produce high quality seedling,promote and apply in the factory seedling production of Amorphophallus konjac,and also provide important reference for study on tissue culture technology of other varieties in Amorphophallus konjac.
出处
《安徽农业科学》
CAS
2021年第16期123-125,152,共4页
Journal of Anhui Agricultural Sciences
基金
玉溪市农业科学院科研项目“作物组培扩繁及配套栽培技术与应用”(YXNKYZP2020)。
关键词
花魔芋
球茎
顶芽
组织培养
快速繁殖
培养基
Amorphophallus konjac
Corm
Apical bud
Tissue culture
Rapid propagation
Medium
