摘要
采用组织切片、H.E和AB.PAS染色技术对不同日龄鳜(Siniperca chuatsi)的幽门盲囊数量、组织学特征以及黏液细胞类型和分布进行观察和描述。结果表明:15日龄鳜在胃肠之间左腹侧有1枝幽门盲囊开始发育;20日龄鳜在肠下方右腹侧出现1簇幽门盲囊;25日龄鳜在胃肠之间右腹侧和肠下方左腹侧各出现1簇幽门盲囊;随着日龄增加,鳜的幽门盲囊数量继续增加。20、30、40、50和60日龄,鳜的幽门盲囊数量分别为(25±2)、(75±2)、(123±4)、(155±7)、(190±5)条。幽门盲囊的长度、肌层厚度及内侧的皱襞数量也随鳜发育日龄而呈现递增趋势。幽门盲囊由多枝汇集成簇,每枝在中部产生2个分支,盲囊顶部比基部皱襞数量少且长度短。幽门盲囊黏液细胞以Ⅱ型为主,Ⅳ型次之,Ⅲ型最少,未见Ⅰ型黏液细胞,盲囊基部黏液细胞数量显著多于顶部。
The number, histological characteristics, types and distribution of mucus cells in the pyloric caecum of Siniperca chuatsi at different developmental age were observed and described by histology sectioning, H.E and AB. PAS stainings. The results showed that at 15 days after hatching, there was a cluster of pyloric caeca began to develop on the left ventral side between the stomach and intestine. At 20 days after hatching, there was a cluster of pyloric caeca that developed on the right ventral side below the intestinal tract, and at 25 days after hatching, there was a cluster of pyloric caeca that developed on the right ventral side between the stomach and intestinal side, and the left ventral side below the intestine, respectively. At the age of 20, 30, 40, 50 and 60 days, the number of pyloric caeca was(25±2),(75±2),(123±4),(155±7) and(190±5), respectively. The number and the length of the folds and the thickness of the muscular layer of pyloric caecum increased with development age. The pyloric caecum was clustered by multiple branches, each of which produced two sub-branches in the middle section. Ⅱ type of mucous cell was widely distributed in the pyloric caecum, followed by type Ⅳ, there was no Ⅰ type mucous cell. The number of mucous cells on the base pyloric caecum was higher than the top part.
作者
赵亮亮
施琳澜
赵金良
周云红
ZHAO Liangliang;SHI Linlan;ZHAO Jinliang;ZHOU Yunhong(Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2021年第4期684-690,共7页
Journal of Shanghai Ocean University
基金
财政部和农业农村部国家现代农业产业技术体系专项(CARS-46)。
关键词
鳜
幽门盲囊
器官发生
组织学
Siniperca chuatsi
pyloric caecum
organogenesis
histology
