摘要
转录因子在真菌环境响应及生理过程中发挥重要的调节作用。本文利用转录组测序(RNA-Seq)技术研究光诱导香菇菌丝转色过程中的转录因子表达变化。转录组测序结果表明,在光照条件下转色的菌丝(313C)与黑暗条件下未转色的菌丝(313W)相比,香菇菌丝中共有68个转录因子基因表达发生变化(48个转录因子上调表达,20个转录因子下调表达);转色的菌丝(313C)与初始未转色的菌丝(118)相比,香菇菌丝中有80个转录因子基因表达发生变化(49个转录因子上调表达,31个转录因子下调表达)。这些差异表达的转录因子包括WD40、MADS-box、MYB和GATA等家族。另外,样品的两两比较中差异表达的转录因子既存在部分重叠,也表现特异性。其中,313C/313W中有14个特异性差异表达转录因子,313C/118中有26个特异差异表达的转录因子。重叠的转录因子有64个,其中有39个表达水平上调,15个表达水平下调。采用实时荧光定量PCR对几个转录因子进行了表达检测,其中部分转录因子的表达趋势与转录组分析基本相同。这些数据为进一步研究香菇转色的转录调控奠定了一定的基础。
Transcription factors play an important role in the regulation of fungal environmental responses and physiological processes. The expression changes of transcription factor genes in light-induced brown mycelial film（BMF） formation were analyzed by the next-generation high-throughput sequencing technology. The results showed that there were 68 differential expressed transcription factor（TF） genes（48 up-regulated genes and 20 down-regulated genes） when comparing the BMF sample 313 C formed under light inducement with white mycelial sample 313 W formed in dark. Whereas, 80 differentially expressed TF genes were obtained（49 up-regulated genes and 31 down-regulated genes） when comparing the sample 313C（with BF） with initial white mycelial sample 118. These TFs belong to the families like WD40, MADS-box, MYB and GATA. The expression of transcription factor genes was different in light-induced BF formation, and there were stage-specific TF genes as well as those overlapping among the three samples. There were 14 TFs between the samples 313 C and 313 W, and 26 between the samples 313 C and 118 being specifically transcibed by the fungus. There were 64 TF genes with overlapping expressional profiles, of which 39 TFs were up-regulated and 15 TFs down-regulated. The results of transcriptome analyses were examined by real-time quantitative PCR. The data of this study will facilitate future functional investigations of the TFs involved in Shiitake mushroom developmental controls.
出处
《菌物学报》
CAS
CSCD
北大核心
2016年第9期1106-1116,共11页
Mycosystema
基金
上海市市级农口系统青年人才成长计划[沪农青字(2015)第1-9号]
国家自然科学基金(31170098)
上海市科委崇明专项(13391912202)~~
关键词
香菇
转色
光线
转录因子
高通量测序
Lentinula edodes
brown film formation
light
transcription factors
high-throughput sequencing
