摘要
以质粒pCAMBIA1301和pBI121为基础构建草菇表达载体pCB。把已克隆的草菇开伞相关基因DNA片段正反向插入pCB,获得pCB opn15和pCB opn39,经测序证实,pCB opn15为正向插入,pCB opn39为反向插入。
<Abstrcat> An expression vector of pCB for Volvariella volvacea was constructed with plasmid pCAMBIA1301and pBI121. The DNA fragment of fruitbody opening related gene (opn) was inserted to plasmid pCB by two orientation. Two recombined plasmids of pCB-opn 15 and pCB-opn 39 were obtained,and the sequencing results showed that pCB-opn 15 was inserted in positive orientation and pCB-opn 39 was inserted in inversion.
出处
《食用菌学报》
2005年第2期9-13,共5页
Acta Edulis Fungi
基金
福建省自然科学基金重点项目"草菇开伞基因克隆及反义基因构建"(编号:B0120002)的部分内容
