摘要
对膜技术在花生蛋白酶解液分离纯化应用的工艺进行了研究。考察了超滤膜截留相对分子质量（1、3、5 kDa）、超滤压力（0.086、0.121、0.157、0.193 MPa）、超滤温度（30、35、40、45℃）、超滤加水量（0.5-5.5倍）对超滤效果的影响,以及考察加水次数（1-12次）对纳滤脱盐效果的影响。结果表明：超滤操作的最适工艺为用截留相对分子质量为1 kDa的超滤膜,在超滤压力0.193MPa、超滤温度45℃的条件下进行恒体积超滤,加水量以加入3倍水为最适,此时短肽透过率可达65.01%,透过液中短肽的相对分子质量主要分布在283-402 Da之间;纳滤操作在纳滤温度为常温（20℃）、纳滤压力为1.5 MPa的条件下进行,采用间歇式恒容纳滤方式,最适加水次数为10次,此时Na＋脱除率为（69.78±0.69）%,短肽损失率仅为2.00%。采用最适的先超滤、后纳滤联用工艺制备的花生短肽液经冻干后,冻干粉对ACE的IC50为0.78 mg/mL,体外抗氧化活性为ORAC值（以Trolox计）（2 359.50±40.43）μmol/g,表明短肽同时具有较强的ACE抑制活性和体外抗氧化活性。
The application of membrane technique in separation and purification of peanut protein enzymatic hydrolysate was studied. The impacts of retention relative molecular weight of uhrafiltration membrane （ 1 kDa, 3 kDa, 5 kDa）, ultrafiltration pressure （0. 086 MPa, 0. 121 MPa, 0. 157 MPa, 0. 193 MPa） ,ultrafiltration temperature （30 ℃ ,35 ℃ ,40 ℃ ,45 ℃ ）and water dosage（0.5 -5.5 times of peanut protein enzymatic hydrolysate） on ultrafiltration effect were discussed, and the influence of water addition times （ 1 - 12 times） on desalination effect of nanofiltration was also discussed. The results showed that the optimal ultrafiltration conditions were obtained as follows： retention relative molecular weight of ultrafihration membrane 1 kDa, ultrafihration pressure 0. 193 MPa, uhrafiltration temperature45 ℃, water dosage 3 times of peanut protein enzymatic hydrolysate with constant volume ultrafiltration. Under these conditions, the transmittance rate of short peptides was up to 65.01%, and the distribution of relative molecular weight of short peptides in the permeate was in the range of 283 -402 Da. The optimal nanofiltration conditions were obtained as follows：nanofiltration temperature room temperature （ 20 ℃ ）, nanofihration pressure 1. 5 MPa, water addition times10 times with interval constant volume nanofihration. Under these conditions, the removal rate of Na was （69.78 ＋ 0.69） %, while the loss rate of short peptides was only 2.00%. The short peptides prepared by the process of first ultrailtration then nanofiltration were freeze -dried into peptides powder,the IC50 value to angiotensin - converting enzyme （ACE） and oxygen radical absorbance capacity （ORAC） value of the peptides powder were 0.78 mg/mL and （2 359.50 ＋ 40.43 ） μmolTmlox/g respectively, which indicated that the short peptides had good ACE inhibitory activity and in vitro antioxidant activity.
出处
《中国油脂》
CAS
CSCD
北大核心
2014年第10期24-29,共6页
China Oils and Fats
基金
广东省科技计划项目(2011B010500006)
"十二五"国家科技支撑计划项目(2012BAD33B10)
深圳市科技计划项目(ZDSY20120619093923525)
深圳职业技术学院校级重点项目(2210k3070013)
广东省高等职业院校珠江学者岗位计划资助项目(2011年度)
关键词
膜分离
超滤
纳滤
酶解液
肽
生物活性
血管紧张素转化酶
抗氧化活性
membrane separation
uhrafiltration
nanofiltration
enzymatic hydrolysate
peptide
bioaetivity
angiotensin - converting enzyme
antioxidant activity
