摘要
目的:研究鲣鱼活性肽酶解工艺,探讨鲣鱼活性肽对小鼠抗疲劳的影响。方法:以鲣鱼活性肽得率为评价指标,采用木瓜蛋白酶、中性蛋白酶、风味蛋白酶、碱性蛋白酶、酸性蛋白酶对鲣鱼鱼糜最佳用酶进行筛选,考察料液比、加酶量、酶解时间和酶解温度对酶解工艺的影响,在单因素实验的基础上,结合响应面法进行优化设计,得到酶解鲣鱼活性肽的最佳工艺。构建递增负荷式游泳训练所致的疲劳小鼠模型,按体质量随机分为安静空白组、运动模型组、阳性对照组、鲣鱼活性肽低剂量组(200 mg/kg,简称低剂量组)、鲣鱼活性肽中剂量组(400 mg/kg,简称中剂量组)、鲣鱼活性肽高剂量组(800 mg/kg,简称高剂量组)。除安静空白组外,其余各组以训练时间为变量进行递增负荷式游泳训练。采用负重力竭游泳实验检测小鼠的负重游泳力竭时间;检测小鼠血清尿素氮(BUN)含量、乳酸(LD)含量、乳酸脱氢酶(LDH)活性以及肝脏丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性。结果:(1)风味蛋白酶酶解鲣鱼活性肽得率均高于中性蛋白酶、木瓜蛋白酶、碱性蛋白酶、酸性蛋白酶,差异有统计学意义(P<0.01或P<0.05);在固定料液比1∶1(g/mL)的基础上,风味蛋白酶酶解优化条件为加酶量4400 U/g,酶解时间6.5 h,酶解温度42℃;该条件下鲣鱼活性肽得率为33.166%。(2)运动模型组小鼠的BUN、LD、MDA含量和LDH、SOD活性均极显著高于安静空白组(P<0.01)。与运动模型组相比,阳性对照组及鲣鱼活性肽低、中、高剂量组均极显著延长小鼠力竭游泳时间(P<0.01),减少运动后小鼠血清BUN、LD和肝脏MDA含量及降低血清LDH活性(P均<0.01),提高肝脏SOD活性(P<0.01)。结论:通过响应面法优化风味蛋白酶酶解鲣鱼活性肽的工艺,验证了鲣鱼活性肽具有减轻力竭游泳小鼠疲劳的作用。
Objective:To optimize the enzymatic hydrolysis technology of bioactive peptide in Katsuwonus pelamis and observe the anti-fatigue effect of bioactive peptide on mice.Methods:With the yield of bioactive peptide in Katsuwonus pelamis as the evaluation index,the papain,neutral protease,flavor protease,alkaline protease and acid protease were used to screen out the best enzymes for Katsuwonus pelamis surimi.The effects of solid-liquid ratio,enzyme amount,enzymatic reaction time and temperature on the enzymatic hydrolysis were investigated.The response surface methodology was designed based on the single factor experiment to obtain the optimal enzymatic hydrolysis condition of bioactive peptide in Katsuwonus pelamis.The fatigue mice model was established by incremental loaded swimming training and the experiment mice were randomly divided into quiet blank group,exercise model group,positive control group,low-,medium-and high-dose of bioactive peptide in Katsuwonus pelamis groups(200,400,800 mg/kg,respectively)according to the body mass.Except for the quiet blank group,the other groups were trained with incremental loaded swimming with the training time as the variable.The loaded swimming test was conducted to detect the exhaustion time of mice.The contents of serum urea nitrogen(BUN)and serum lactic acid(LD),serum lactic dehydrogenase(LDH)activity,liver malondialdehyde(MDA)content and liver superoxide dismutase(SOD)activity were measured with corresponding kits.Results:1)The yield of bioactive peptide in Katsuwonus pelamis hydrolyzed by flavor protease was statistically higher than that of neutral protease,papain,alkaline protease and acid protease(P<0.01 or P<0.05).When the solid-liquid ratio was fixed at 1∶1(g/mL),the optimal conditions of flavor protease hydrolysis were enzyme amount 4400 U/g,enzymatic time 6.5 h and temperature 42℃.Under these conditions,the yield of bioactive peptide in Katsuwonus pelamis was 33.166%.2)The contents of BUN,LD,MDA,and the activities of LDH,SOD in the exercise model group were s
作者
李守江
孔祥佳
刘智禹
赵峰
苏永昌
陈晓婷
LI Shoujiang;KONG Xiangjia;LIU Zhiyu;ZHAO Feng;SU Yongchang;CHEN Xiaoting(Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Xiamen,Fujian 361013,China;Fisheries Research Institute of Fujian,Xiamen,Fujian 361013,China)
出处
《康复学报》
CSCD
2022年第5期407-418,共12页
Rehabilitation Medicine
基金
国家重点研发计划课题(2018YFD0901102)
福建省海洋生物增养殖与高值化利用重点实验室开放课题(2021fjscq10)。
关键词
鲣鱼
活性肽
酶解
响应面法
抗疲劳
Katsuwonus pelamis
bioactive peptide
enzymatic hydrolysis
response surface methodology
anti-fatigue
