摘要
探讨纳滤技术浓缩纯化玉米肽的过程中肽的醒酒活性的变化。本研究选用截留分子质量160D和360D的两种纳滤膜,对pH8.0玉米肽液(Mw<5kD)进行浓缩纯化,比较两种膜对其中主要成分的传质效果,并进一步探讨透析过滤过程对玉米肽醒酒活性的影响。结果表明,玉米肽液浓缩过程中,相对于360D纳滤膜,160D纳滤膜对肽类、氨基酸和Na+均具有较低的传质效率,但其对玉米肽的截留率却在88.80%以上,脱盐效果与360D膜相当,经3次透析后,脱盐率达94.6%,且160D纳滤膜在透析过程中很好地保持玉米肽对乙醇脱氢酶(ADH)的激活作用。结论:宜选用160D纳滤膜对玉米肽液进行浓缩纯化,透析过滤3次。
Nanofiltration membranes（NFM）with molecular weight cut off（MWCO）160 D and 360 D were used to concentrate and purify corn protein hydrolysate of MW5 kD at pH 8.0.The mass transfer efficiencies of peptides,amino acids and sodium ion（Na＋）in concentration process and the changes in hydroxyl free radical scavenging activity and antialcoholism activity of corn peptides（CPs）in dialysis process were evaluated.The results showed that although 160 NFM exhibited lower transmission/ rejection rates of CPs,amino acids and Na＋as compared to 360 D NFM,the rejection rate of CPs by 160 D NFM was above 88. 80%and it provided desalting effect similar to that of 360 D NFM and yielded a desalting efficiency of 94.6%after triple repeated dialysis.Moreover,the activating effect of corn peptides on alcohol dehydrogenase（ADH）was kept very well in 160 D NFM dialysis.In general,160 D NFM is suitable to be used for the concentration and purification of corn protein hydrolysate and the number of repeated dialysis should be no more than 3.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第8期10-14,共5页
Food Science
基金
国家自然科学基金项目(30972043)
国家"863"计划项目(2008AA10Z314)
关键词
纳滤
玉米肽
醒酒
透析
脱盐
乙醇脱氢酶
nanofiltration（NF）
corn peptides
antialcoholism
dialysis
desalination
alcohol dehydrogenase（ADH）
