摘要
背景:骨折动物模型无疑是研究骨折愈合的基石,其中开放截骨模型与闭合骨折模型是较成熟的两种长骨干骨折模型。就作者所查文献,未见关于大鼠两种模型比较研究的报道。目的:比较大鼠股骨开放截骨模型与闭合骨折模型的制作过程,并通过X射线摄片比较二者的骨折愈合情况。设计、时间及地点:随机对照动物实验,于2007-01/05在苏州大学附属第二医院骨科实验室完成。材料:SPF级雄性SD大鼠40只,体质量(220±30)g,随机分为开放截骨模型组与闭合骨折模型组,每组20只。电动摆锯;自行改良设计的闭合骨折模型打击器。方法:开放组行开放截骨术造成股骨中段横行骨折,逆行克氏针固定;闭合组行逆行克氏针固后,使用造模支架致股骨中段闭合骨折。主要观察指标:比较两组的平均造模时间,造模成功率及术后2,3,4,8周骨折愈合的X射线情况。结果:开放截骨模型每只造模成功率90%,平均造摸时间为17min,骨折畸形愈合及不愈合率40%;闭合骨折模型每只造模成功率85%,平均造模时间12min,未见畸形愈合及不愈合。术后2,3,4周X射线评分开放组均低于闭合组(P<0.05),8周时开放组X射线评分仍较闭合组低,但差异无显著性意义(P>0.05)。结论:开放截骨模型造模时间长,骨折畸形愈合不愈合发生率高,近中期骨折愈合明显滞后,不适于近中期骨折愈合的研究。
BACKGROUND： There is no doubt that animal model of fracture is the basis of fracture healing research. The fracture animal mode is divided into open osteotomy model and closed fracture model. There is no research about the difference between two kinds of fracture rat models. OBJECTIVE： To compare the modeling process between open osteotomy model and closed fracture model in rats, and to compare fracture healing using radiograph. DESIGN, TIME AND SETTING： The randomized, controlled animal experiment was performed at the Laboratory of Department of Orthopaedics, Second Affiliated Hospital, Soochow University from January to May 2007. MATERIALS： A total of 40 SPF male Sprague Dawley rats, weighing （220±30） g, were randomly divided into an open osteotomy model group and a closed fracture model group, with 20 rats for each group. Electric swing saw was a kind of self-made percussion device for closed fracture models. METHODS： All rats in the open osteotomy model group received open osteotomy to create transverse fracture of the middle femur, and fixed using Kirschner wire. Rats in the closed fracture model group received retrograde Kirschner wire fixation, and midshaft femoral closed fracture induced by model establishment stent. MAIN OUTCOME MEASURES： Mean model establishment time; Successful rate of model establishment; Radiography of fracture healing at 2, 3, 4 and 8 weeks after surgery. RESULTS： Success rate of open osteotomy model establishment was 90%. Average modeling time was 17 minutes. Rate of malunion and nonunion was 40%. Successful rate of each closed fracture models was 85%, with average modeling time was 12 minutes. There was no malunion and nonunion. At 2, 3 and 4 weeks after surgery, X-ray score was lower in the open osteotomy model group compared with the closed fracture model group （P 〈 0.05）. At 8 weeks, X-ray score was still lower in the open osteotomy model group than in the closed fracture model group （P 〉 0.05）. CONCLUSION： Model establishment of open osteotomy
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第46期9071-9075,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国防预先研究项目(61601030500)~~
