摘要
目的 探讨实验性骨质疏松状态对骨基质明胶修复骨缺损成骨效能的影响。方法 将 6 8只雌性SD大鼠随机分为去卵巢组和对照组。 90d后两组各抽取 10只测定骨密度以确定骨质疏松模型的建立。其余 4 8只大鼠制备直径 8mm颅骨极量骨缺损并同期植入骨基质明胶颗粒。植入术后 2 1和 5 6d两组每次分别处死 12只大鼠 ,通过不脱钙组织磨片、四环素荧光示踪及骨组织计量学动态参数测定、扫描电镜和X射线显微分析技术观察骨缺损区修复情况。结果 骨密度测定指标显示 ,去卵巢 90d后骨质疏松状态建立。骨基质明胶植入术后 2 1d ,均有新骨在两实验组骨缺损内形成 ,去卵巢组新骨形成较少 ,四环素标记成骨区域较稀疏。植入术后 5 6d ,对照组骨缺损基本修复 ,去卵巢组骨缺损部分区域为纤维结缔组织充填。植入术后 2 1d、5 6d荧光双标间距和平均矿化沉积速率均较对照组低 ,差异有统计学意义 (P <0 0 1)。骨缺损区新生骨痂的钙磷比值定量分析 ,去卵巢组均低于对照组 ,差异有统计学意义 (2 1d :0 4 892± 0 0 342、0 5 4 75± 0 0 2 0 4 ,P <0 0 1;5 6d :1 0 95 0± 0 0 4 37、1 195 5± 0 0 4 6 3,P <0 0 1)。结论 实验性骨质疏松降低了骨基质明胶的成骨效能。
Objective To study the influence of experimentally osteoporosis to osteogenic efficiency of bone matrix gelatin(BMG)implanted into the calvarial defects of rats. Methods Sixty |eight female SD rats of 12±1 weeks were randomly divided into two groups with 34 rats in each group.The ovaries were excised in the ovariectomized group(OVG).The control group underwent sham surgery.Ninety days after ovariectomy,10 rats from each group were examined to ensure the formation of postmenopausal osteoporosis by measuring bone density of the femur with single photon absorptionmetric measurements.A critical |sized(8 mm in diameter)calvarial defect was created on the rest of 48 rats.Bone matrix gelatin was implanted to the defect.The rats were scarified at the 21st and 56th day after surgery respectively.The new bone forming capability of BMG was evaluated with undecalcified histological observation,tetracycline fluorescence marker,quantitative bone histomorphometry,scanning electron microscopy and X |ray spectrometry. Results At 90th day after ovariectomy,bone density of OVG showed very significant difference compared with the control group (0 315±0 015)g/cm +2vs[(0 347±0 017)g/cm +2, P <0 01].At the 21st day following the implantation operation,new bone formed within the bone defects in both groups.The amount of new bone in OVG was lower than the control group.The tetracycline |labeled region in the bone defect was sparser in the OVG.At the 56th day,the bone defects healed mostly in the control group but fibrous tissue filled parts of bone defect in the OVG.The distance between two fluorescent lines of incorporated tetracycline and the mean mineralization deposition were significantly lower in the OVG than the control at the 21 st day and 56th day.Mineralization of callus in OVG was inferior.Significant difference was found between the OVG and the control group in the calcium to phosphate ratio of callus in bone defects at the two time |points. Conclusions Experimentally induced osteoporosis depressed os
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2005年第1期24-28,共5页
Chinese Journal of Plastic Surgery
