摘要
目的 研究染料木黄酮对去势大鼠股骨远端形态计量学参数的影响 ,为染料木黄酮防治骨质疏松提供理论依据。方法 雌性Wistar大鼠 4 7只 ,按体重随机分为 6组 :假手术组、去势对照组、去势 +雌激素组 (2 0 μg/kg体重 )、去势 +染料木黄酮组 (染料木黄桐剂量分别为 2 5、5 0、10 0mg/kg体重 )。饲养 3个月后处死 ,测定股骨形态计量学参数。结果 去势组与假手术组比较 ,骨小梁体积、平均骨小梁板密度和厚度减少 ,平均骨小梁板间隙和类骨质宽度增加 ,矿化延迟时间和类骨质成熟时间延长 ,且差异均具有显著性 (P <0. 0 5 )。染料木黄酮各组与去势组比较 ,骨小梁体积和平均骨小梁板厚度增加 ,平均骨小梁板间隙变窄 ,矿化延迟时间和类骨质成熟时间缩短 ,且差异均有显著性 (P <0 . 0 5 )。结论 染料木黄酮有促进骨形成 ,减少切除卵巢后骨量丢失的作用。
Objective To study the effects of genistein on bone histomorphometric parameters of femoral distal diaphysis in ovariectomized rats. Methods Wistar female rats were randomly allocated to six groups:the sham-operated, the OVX+estrogen(20 μg/kg weight), the OVX+genistein (25,50,100 mg/kg weight, separately), and OVX group. Having been fed for 13 weeks, the rats were sacrificed, and histomorphometric and dynamic parameters of the rats were analysed. Results The trabecular bone volume, the mean trabecular plate density and mean trabecular plate thickness of OVX rats significantly decreased, but their mean trabecular plate space and mean osteoid surface width increased significantly, and mineralization lag time and osteoid maturation period were significamtly prolonged, compared with sham-operated group. After three months of genistein supplementation to ovariectomized rats, the trabecular bone volume, mean osteoid surface width, mineralization lag time, and osteoid maturation period were sigmificamtly improved. Conclusions Genistein reduces bone loss in ovariectomized rats.
出处
《中国骨质疏松杂志》
CAS
CSCD
2005年第1期49-51,共3页
Chinese Journal of Osteoporosis
