摘要
背景:以往制备骨关节模型的方法成模时间较长。目的:建立一个理想的兔膝关节软骨缺损动物模型。设计、时间及地点:随机分组设计,对照动物实验,于2007-03/2008-10在广州市红十字会医院创伤外科研究所完成。材料:用新西兰大白兔16只,雌雄不限,体质量3.2～4.0kg。方法:以6g/L戊巴比妥钠行耳缘静脉注射麻醉下,通过在实验兔后腿关节的股骨髁关节面部位用电动骨钻,在控制其直径4.5mm,深度为3mm,造成其关节软骨的缺损,制模后2,6,12,18周取出其股骨髁。主要观察指标:用大体观察、组织学染色共聚焦显微镜观察缺损部位软骨修复情况。结果:实验兔后腿关节的股骨髁关节面的关节软骨缺损后,在制模后2,6周缺损部位凹陷明显,只有少许肉芽组织和纤维组织生长填充,未见软骨细胞爬行生长,12,18周见缺损部位以纤维组织增生为主,底部及边缘可见骨组织和软骨增生,但是镜下形态仍与正常软骨组织区别较大,表面仍未见软骨细胞爬行生长。结论:实验建立的兔膝关节软骨缺损模型成模时间短,且缺损部位不能通过自我修复达到自愈。
BACKGROUND： The modeling period of bone joint is rather long by previous methods. OBJECTIVE： To establish an ideal rabbit models of knee articular cartilage defect. DESIGN, TIME AND SETTING： Randomized grouping and controlled animal experiment were performed at the Institute of Traumatic Surgery, Guangzhou Red Cross Hospital between March 2007 and October 2008. MATERIALS： Sixteen New Zealand rabbits, irrespective of gender, weighing 3.2 4.0 kg, were selected. METHODS： Under anesthesia by ear margin intravenous injection of 6 g/L sodium pentobarbital, a defect, 4.5 mm diameter, 3 mm depth, was drilled at the condyles of femur of the hind legs using an electric bone drill. The condyles of femur were harvested on weeks 2, 6, 12 and 18. lAIN OUTCOME MEASURES： Cartilage repair at defect site was observed by gross observation and histological staining under confocal microscopy. RESULTS： The collapse was obvious at weeks 2 and 6 after modeling, with only few granulation tissues and fibrous tissues. No chondrocytes were observed. At weeks 12 and 18, fibrous tissues were found at the defect site, and bone tissue and cartilage proliferation was observed at the bottom and margin. However, it was greatly different from normal cartilage. Moreover, there was still no chondrocytes. CONCLUSION： The results show that the modeling period is short by this method, and the defect could not repair by itself.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第2期280-283,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广州市科技攻关项目(2006Z3-E0371)
广州市医药卫生科技重点项目(2006-ZDi-03
2006-ZDi-04)
广州市科技局项目(2008JI-CI21)~~
