摘要
①目的 研究p16、Rb和rasp21 蛋白在子宫内膜癌中的表达及与雌激素受体(ER)和孕激素受体(PR)表达的关系,探讨子宫内膜癌发生、发展过程中存在着的多因素、多步骤、多阶段的癌变机制。②方法 应用免疫组化SABC法检测21例正常子宫内膜、19例子宫内膜上皮内瘤样病变(EIN)及45例子宫内膜癌组织中p16、Rb和rasp21蛋白表达,同时检测子宫内膜癌组织中ER和PR的表达情况。③结果 子宫内膜癌中p16 和Rb蛋白的阳性表达率低于正常子宫内膜(χ2=9.03、4.37,P<0.01、0.05),正常子宫内膜中rasp21蛋白阳性表达率低于子宫内膜癌和EIN(χ2=10.99、3.87,P<0.01、0.05),其表达与子宫内膜癌的临床分期、肌层浸润及组织分级有关(P=0.015,χ2=7.29、6.65, P<0.01)。子宫内膜癌组织中,p16与Rb蛋白的表达呈正相关(r=0.42,P<0.01),rasp21蛋白与ER、PR的表达呈负相关(r=-0.46、-0.42,P<0.01)。④结论 p16、Rb和rasp21 基因均参与子宫内膜癌的发生,rasp21基因的异常表达是子宫内膜癌发生的早期事件,并与ER和PR有协同作用,为子宫内膜癌恶性进展和预后的不利因素。
Objective To study the expressions of p16, Rb and rasp21 proteins in endometrial carcinoma and the relationship between the expressions and estrogen receptor（ER） and progestagen receptor （PR）,and to demonstrate the stepwise abnormalities of multiple factors in the carcinogenesis and development of endometrial carcinoma. Methods Expressions of p16, Rb, rasp21, ER and PR were examined immunohistochemically in 21 normal endometrium, 19 endometrial intraepitheliaI neoplasia （EIN） and 45 endometrial carcinoma. Results The positive expression rates of p16 and Rb protein in normal endometrium were higher than those in endometrial carcinoma（χ^2=9.03,4.37;P〈0.01, 0.05） ; while those of rasp21 protein in normal endometrium were lower than t hose of endometrial carcinoma and EIN （χ^2=10. 99,3. 87 ; P 〈 0. 01,0. 05 ）. The expression of rasp21 protein showed significant correlation with clinical stages, myometrial invasion and histological grade of endometrial carcinoma（P = 0.015; χ^2=7.29,6.65;P〈0.01）. In endometrial carcinoma, the expressions of p16 and Rb protein were positively correlated（r=0.42, P〈0.01）, while rasp21 and ER, PR were inversely correlated（r= 0. 46,-0. 42;P〈0. 01）. Conclusion pl6,Rb and rasp21 proteins might play some roles in the carcinogenesis of endometrial carcinoma. The abnormality of rasp21 protein, together with ER and PR, is the early stage of carcinogcnesis and the negative factor of progression and prognosis of endometrial carcinoma.
出处
《齐鲁医学杂志》
2005年第2期139-141,共3页
Medical Journal of Qilu
