摘要
目的 :本实验检测原癌基因 H-ras的转录和翻译水平在妊娠早期小鼠子宫内膜的表达规律。方法 :采用反转录聚合酶链反应 ( RT-PCR)、免疫转移印迹 ( Western-blot)和免疫斑点印迹 ( Dot-blot)法。结果 :原癌基因 H-ras在妊娠早期小鼠子宫内膜的表达呈现两个峰值 ,分别为妊娠的第 1和第 6、7天。结论 :提示原癌基因
Objective: To study the expression of protooncogene H-ras transcription and translation in the endometrium of early pregnant mouse. Methods: (1)The expression of H-ras mRNA was analyzed by means of RT-PCR. (2) The expression of H-ras proteins was analyzed by means of immunoblot and western-blot. Results: The expression of H-ras mRNA in the endometrium of early pregnant mouse showed two peaks. The first peak occurred on day 1, the second peak on day 6 and day 7 of pregnancy. The expression of H-ras protein had the similar pattern. Conclusion: It indicated that gene H-ras might by involved in the signal transduction of the embryo implantation process and the early differentiation and proliferation of embryo.
出处
《生殖医学杂志》
CAS
2002年第3期163-167,共5页
Journal of Reproductive Medicine
