摘要
目的探讨 p16蛋白表达及DNA含量在小儿急性淋巴细胞白血病 (ALL)中的临床意义。方法应用S_P免疫组化法检测31例ALL患儿的 p16蛋白表达水平 ,流式细胞术检测白血病细胞DNA含量。结果31例ALL的p16蛋白表达缺失率为54.8 % (17/31例 ) ,p16蛋白缺失与高白细胞数 (>2.5×109/L)、肝脾肿大等高危临床特征显著相关 ;高危型ALL的p16基因缺失率 (70.5 % )远高于标危型ALL(35.7 % ) ,两组比较差异有显著性 (P=0.0459)。小儿ALL异倍体发生率为41.8 % ,p16蛋白表达阴性者17例中DNA异倍体9例 ,p16蛋白表达阳性者中异倍体4例 ,两组差异无显著性 (P=0.2749)。结论p16蛋白失活在小儿ALL的发病过程中起重要作用 ,p16蛋白表达阴性者DNA异倍体的发生率高 ,与临床高危因素有关 ,p16蛋白缺失者可能与ALL复发有关。
Objective The p16protein deletion of tumor suppressor gene p16will lead to a loss of cell function in G1/S cell cycle position.Leukemia may result from aberrant DNA duplication as a result of aberrant genome.The clinical and biological implications of this feature were analyzed by studying p16protein expression and DNA content in childhood ALL in order to explore the correlations between them and its association with clinical manifestations and outcome.Methods Bone marrow samples were obtained from31children with ALL including one with a relapsing ALL(18males and13females,aged one year and a month to14years)before chemotherapy.DNA content of the cells was determined by flow cytometry after propidium iodide staining.A diˉrect smear was prepared from0.2ml bone marrow blood cells for the routine S_P immunohistochemical method to determine the expression of p16protein.Results Among31cases,17cases were clinically classified as high risk ALL,14as standard risk ALL and11as control group(aged2years and3months to11years).The negative exˉpression rate of p16protein in childhood ALL patients was54.8%(17/31).The high_risk clinical features including hepatomegaly,splenomegaly and high white blood cell count(>2.5×10 9 /L)were correlated with deletion of p16protein expression.The negative expression rate of p16protein in high_risk group of ALL(70.5%)was signifiˉcantly higher than that in standard risk group(35.7%)(P=0.0459).The DNA aneuploidy rate in childhood ALL patients was41.8%.The DNA aneuploidy of ALL was found in9of17samples with deletion of p16protein and in4of14samples with positive expression of p16,respectively(P=0.2749).Conclusions The negative expression of p16protein might play an important role in the pathogenesis of ALL.The levels of p16protein expression correlate with such factors as the high_risk features and the DNA aneuploid.It may serve as one of the indicators to predict the prognosis of children with ALL.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2005年第2期87-89,共3页
Journal of Clinical Pediatrics
