摘要
植物多糖是具有保健功能的生物活性成分,黄秋葵鲜嫩果具有很高的多糖含量。对黄秋葵多糖含量进行全基因组关联分析(Genome-wide Association Study, GWAS),挖掘相关基因位点,可以为利用分子育种手段培育高多糖黄秋葵新品种奠定理论和材料基础。对180份黄秋葵种质进行GBS建库测序(Genotyping by sequencing, GBS),结合多环境下黄秋葵多糖含量的表型数据进行全基因组范围的关联分析,以明确与多糖含量显著关联的SNP位点,挖掘位点附近可能与多糖含量相关的基因。通过4个环境共检测到46个显著关联的SNPs位点,其中Cluster1671-67622和Cluster86-210279位点至少在两个环境下稳定出现。在这2个显著关联的位点附近的100 Mb区域内发掘出黄秋葵多糖含量性状关系密切的候选基因,经过分析筛选确定UGT-糖基转移酶和MYB转录因子为候选基因。UGT-糖基转移酶和MYB转录因子与黄秋葵嫩果中多糖的合成、积累和分解紧密相关。本研究结果为阐明多糖的遗传调控机制提供了新的视角,亦为黄秋葵嫩果多糖含量的遗传改良奠定了一定的理论基础。
Plant polysaccharide is a biological active component with health care function. The fresh and tender fruit of okra has a high content of polysaccharide. Genome-wide Association Study (GWAS) on the polysaccharide content of okra and the mining of relevant gene loci can lay a theoretical and material foundation for the cultivation of new varieties of okra with high polysaccharide by molecular breeding methods. Based on the accurate determination of polysaccharide content of 180 okra Germplasms in multi environment, the whole genome sequencing (Genotyping by sequencing, GBS) of the tested materials was carried out, and the genome-wide association analysis was carried out combined with the phenotypic data of polysaccharide content in multi environment, to identify the SNP sites significantly associated with polysaccharide content. Meanwhile, excellent genes which may be related to the content of polysaccharide near the sites were mined. A total of 46 SNPs were detected in four environments, and two of them were selected as candidate sites. In the 100 Mb region near these two significant loci, candidate genes closely related to the polysaccharide content traits of okra were found. UGT-glycosyltransferase and MYB transcription factor were finally identified as candidate genes. UGT-glycosyltransferase and MYB transcription factor are closely related to the synthesis, accumulation, and decomposition of polysaccharides in okra tender fruit. The results of this study provided a new perspective to clarify the genetic regulation mechanism of polysaccharides, and laid a theoretical basis for the genetic improvement of polysaccharide content in okra tender fruit.
出处
《植物学研究》
2021年第6期749-760,共12页
Botanical Research
