摘要
葡聚糖是大麦籽粒的一个重要品质性状,其含量高低影响大麦啤用、饲用和食用品质。虽然有关大麦β-葡聚糖合成的相关基因已有报道,但关于大麦籽粒β-葡聚糖积累的遗传调控机制仍不十分清楚。本研究以前期收集的全球119份大麦基因型为材料,种植在土壤与气候条件有一定差异的两试点,利用混合线性模型(MLM)和一般线性模型(GLM)对不同大麦材料籽粒β-葡聚糖含量进行GWAS分析。结果表明,大麦籽粒β-葡聚糖含量的基因型差异显著,其在2个环境下的广义遗传力为73.9%。利用MLM和GLM模型分别检测到8个和40个显著位点,合并2个模型重叠位点后共得到44个显著位点,其中HORVU5Hr1G022710基因在2个模型、2个地点均被鉴定到,故被认为是与β-葡聚糖含量显著相关的候选基因。2个模型中最佳等位基因数与β-葡聚糖含量均呈显著正相关。此外,基于基因注释,共鉴定到10个与糖合成、转运及分解相关的酶类基因,这些基因可能与籽粒中β-葡聚糖的合成、积累和分解紧密相关。本研究结果为阐明β-葡聚糖的遗传调控机制提供了新的视角,亦为大麦籽粒β-葡聚糖的遗传改良奠定了一定的理论基础。
β-glucan is an important trait in barley,as its content greatly affects the quality in the applications of malting,feeding,and food.Although the genes associated withβ-glucan synthesis have been reported,genetic regulation ofβ-glucan accumulation in barley grains is still unclear.In this study,genome-wide association study(GWAS)with mixed linear model(MLM)and general linear model(GLM)was performed to analyze the grainβ-glucan content of 119 barley germplasms collected from worldwide previously,which were planted at two plots with certain differences in soil and climate conditions.The results showedβ-glucan content in barley grains was significantly different in genotypes and the heritability ofβ-glucan was 73.9%in two environments.There were eight and 40 loci for grainβ-glucan content detected by MLM and GLM,respectively.A total of 44 loci were obtained by combining the same loci of the two models.HORVU5Hr1G022710 gene identified in both models and sites was considered as a putative candidate gene significantly associated withβ-glucan content.Significantly positive correlation was detected between grainβ-glucan content and the number of favorable alleles in both models.In addition,10 enzymatic genes related to sugar synthesis,transport and decomposition were identified based on gene annotations.These genes may significantly relate toβ-glucan synthesis,accumulation and hydrolysis.The results provided a new insight into the genetic regulation ofβ-glucan accumulation and laid a foundation for the genetic improvement breeding of barley seedβ-glucan.
作者
耿腊
黄业昌
李梦迪
谢尚耿
叶玲珍
张国平
GENG La;HUANG Ye-Chang;LI Meng-Di;XIE Shang-Geng;YE Ling-Zhen;ZHANG Guo-Ping(Institute of Crop Science,Zhejiang University,Hangzhou 310058,Zhejiang,China;Wenzhou Vocational College of Science and Technology,Wenzhou 325000,Zhejiang,China;New Rural Development Institute,Zhejiang University,Hangzhou 310058,Zhejiang,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第7期1205-1214,共10页
Acta Agronomica Sinica
基金
浙江省自然科学基金项目(LGN20C130007)
国家自然科学基金项目(31701411)
国家现代农业产业技术体系建设专项(CARS-05)资助。
