摘要
为了研究黄秋葵(Hibiscus esculentus L.)果实老化的机理,测定了其发育过程和采后纤维素含量,显微观察了纤维素在细胞结构中的分布。结果表明,黄秋葵果实老化过程中纤维素含量及在细胞壁的分布大量增加,推测纤维素增加是果实老化的主导因素。从黄秋葵果实转录组测序的RNA-seq数据库中筛选得到9条功能注释为CESA基因家族的片段序列,克隆获得黄秋葵纤维素合酶基因家族CESA1、CESA2、CES43、CESA4、CESA6、CESA7、CES4A等7个基因全长及CESA5、CES4A的片段。通过荧光定量PCR分析这9个基因的表达,结果表明HeCESA1、HeCESA3、He CESA6表达模式相同且其蛋白具有初生细胞壁特异性CESA的保守序列CQIC和SVICEXWF基序;HeCESA4、HeCESA8、HeCESA7的表达模式基本一致,并且在果实发育过程中和采后常温贮藏中纤维素显著增加时表达量显著上调,且远远高于同一时期其他CESA。由此推测HeCESA4、HeCES48和HeCESA7在黄秋葵果实衰老过程纤维素合成中起重要作用。
In order to study the aging mechanism of Hibiscus esculentus fruit,the changes of cellulose content were measured during fruit development and postharvest period,and the distribution of cell structures were observed by microscope. The results showed that during the aging process of H. esculentus fruit,the content of cellulose increased greatly. It was suggested that the increase of cellulose was the main factor of fruit aging. Moreover,nine fragments annotated as CESA gene family were screened from the RNA-seq database of H. esculentus fruit,then the full-length cDNA sequences of CESA1,CESA2,CESA3,CESA4,CESA6,CESA7,CESA8 genes and CESA5 and CESA9 gene fragments were cloned. Expression of these nine genes of cellulose synthase gene family in H. esculentus was analyzed by fluorescence quantitative PCR. The results showed HeCESA1,He CESA3 and He CESA6 had the same expression pattern and conserved sequences CQIC and SVICEXWF motifs of primary cell wall specific CESAs. HeCESA4,HeCESA8 and HeCESA7 had the same expression pattern,and their expression levels were significantly up-regulated during fruit development and postharvest storage when cellulose increased significantly,which was much higher than that of other CESA genes at the same period. It can be inferred that HeCESA4,HeCESA8 and HeCESA7 play an important role in cellulose synthesis of H. esculentus fruit during senescence.
作者
李永平
陈敏氡
刘建汀
曾美娟
朱海生
温庆放
LI Yongping;CHEN Mindong;LIU Jianting;Zeng Meijuan;ZHU Haisheng;WEN Qingfang(Fujian Key Laboratory of Vegetable Genetics and Breeding/Crops Research Institute,Fujian Academy of Agricultural Sciences/Vegetable Research Center,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第1期73-85,共13页
Acta Horticulturae Sinica
基金
福建省属公益类科研院所基本科研专项(2019R1026-9)
国家现代农业产业技术体系建设专项资金项目(CARS-23-G-53)
福建省农业科学院科技创新平台专项(CXPT202001)。
关键词
黄秋葵
老化
纤维素
纤维素合酶基因
Hibiscus esculentus
aging
cellulose
cellulose synthase gene
