摘要
目的:研究抗体筛查细胞在保存期内M抗原、Fya抗原和P1抗原的稳定性变化。方法:应用2个不同厂家的抗体筛查细胞通过倍比稀释法进行单克隆抗体标化(IgG-M/Fya/P1抗体)。收集抗-M/Fya/P1抗体阳性标本,制备人源抗体血清。自配1%试剂红细胞(10例O型且M/Fya/P1抗原阳性志愿者)验证标化单克隆抗体和人源抗体的稳定性。在有效期最后30 d内,采用微柱凝集法,使用标化单克隆抗体和人源抗体对2厂家抗体筛查细胞(试剂1和2)M/Fya/P1抗原的抗原性进行监测。结果:试剂1,M和P1抗原在第21天及Fya抗原在第28天与第0天比较,凝聚强度结果差异有统计学意义(P<0.05);M抗原于第28天及P1抗原于第21天出现无法检出现象。试剂2,M和P1抗原在第21天及Fya抗原在第28天出现凝聚强度差异(P<0.05);M和P1抗原第28天出现无法检出现象。结论:抗体筛查细胞血型抗原稳定性在有效期内逐渐降低,建议抗体筛查细胞保存期间进行质量监测。
Objective:To study the stability changes of M,Fya and P1 antigens in antibody screening cells during the storage period.Methods:Monoclonal antibody standardization(IgG-M/Fya/P1 antibody)was performed by doubling dilution using antibody screening cells from two different manufacturers.The positive specimens of anti-M/Fya/P1 antibody in our department were collected to prepare human antibody serum.Self-prepared 1%reagent red blood cells(10 volunteers with O type and positive for M/Fya/P1 antigen)were used to verify the stability of standardized monoclonal antibodies and human antibodies.During the last 30 days of validity,the M/Fya/P1 antigen antigenicity of the two manufacturers’antibody screening cells(Reagent 1 and Reagent 2)was monitored by microcolumn agglutination and using standardized monoclonal antibodies and human antibodies.Results:For reagent 1,compared with day 0,the results of aggregation strength of M and P1 antigens on day 21 and Fya antigen on day 28 were significantly different(P<0.05).The M antigen was undetectable on the 28 th day and the P1 antigen on the 21 st day.For reagent 2,the aggregation intensity of M and P1 antigens was different on the 21 st day and the Fya antigen on the 28 th day(P<0.05).The M and P1 antigens were undetectable on day 28.Conclusion:The stability of antibody screening cells blood group antigen gradually decreased during the validity period.It is recommended to monitor the quality of antibody screening cells during storage.
作者
卢燕君
晁艳
周厚全
何耀宗
高云龙
王杰婷
吴新忠
梁铮
LU Yanjun;CHAO Yan;ZHOU Houquan;HE Yaozong;GAO Yunlong;WANG Jieting;WU Xinzhong;LIANG Zheng(Department of Clinical Laboratory,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou,510120,China;School of Basic Medicine,Guangzhou University of Chinese Medicine)
出处
《临床血液学杂志》
CAS
2022年第12期841-845,共5页
Journal of Clinical Hematology
关键词
抗体筛查细胞
稳定性
微柱凝集法
人源抗体
单克隆抗体
antibody screening cells
stability
column agglutination
human antibody
monoclonal antibody
