摘要
Objective:Red blood cells are the predominant blood cell component utilized in transfusion therapy.Nevertheless,prolonged storage and the buildup of metabolites can lead to alterations in the structure and function of the plasma membrane of RBCs,thereby impacting their quality.This study aimed to comprehensively examine the evolving dynamics of erythrocyte plasma membrane structure and function under varying storage conditions.Methods:A two-factor,three-level experimental design was employed to investigate the impact of storage temperature(-4℃,4℃,and 10℃)on the composition of erythrocyte membrane phospholipids.High performance liquid chromatography was utilized to analyze the phospholipid composition at 1,7,and 14 d of storage.Additionally,the osmotic fragility of the erythrocyte membrane was assessed using the NaCl solution induction method,and the effective oxygen carrying capacity and oxygen affinity were determined using a blood oxygen analyzer.Results:The findings indicated that the integrity of erythrocyte membranes could be adequately preserved at a temperature of-4℃.In contrast to the groups stored at 4℃and 10℃,there were no notable alterations in the phospholipid composition of erythrocyte membranes and related functional indicators at various time points in the-4℃group.The erythrocyte membrane osmotic fragility in the-4℃group was(0.49±0.05)%,indicating sustained lower levels and suggesting the effective maintenance of cell membrane integrity.Storing erythrocytes at-4℃did not have any detrimental impact on their ability to transport oxygen,as evidenced by the maintenance of normal effective oxygen carrying capacity(29.08±1.59)ml and oxygen affinity(5.33±0.48)mmHg.Conclusion:The findings indicate that erythrocytes can be stored at-4℃for an extended period,with shorter stability observed at 4℃and 10℃.These results offer insights for optimizing the storage duration of erythrocyte products for specific applications.Furthermore,this research holds relevance for informing the develop
