摘要
马铃薯A病毒(Potato virus A,PVA)是马铃薯生产上较常见的病毒之一,植株感病症状与品种、病毒株系和环境条件有关。为研究PVA不同分离物对马铃薯的致病力,将3种遗传背景不同的PVA分离物纯毒源,分别接种到指示植物‘黄花烟’和马铃薯品种‘克新13号’上,应用qRT-PCR检测技术进行病毒侵染鉴定,并观察其致病性差异。结果表明,3种PVA分离物均可成功侵染‘黄花烟’和‘克新13号’,通过接种后‘黄花烟’叶片的症状表现,推断3种分离物的致病性具有一定差异。在马铃薯上分离物2017-171致病力表现为最强,不仅在植株体内病毒含量最高,可致使叶片出现叶缘皱缩、轻花叶,还能导致马铃薯块茎严重裂口、芽眼变深;其次是分离物KB117,病毒侵染速度较快但病毒含量低,植株症状相对明显,叶缘变尖、波浪状皱缩,块茎发生畸形且芽眼变深;最后是分离物2017-176,植株仅个别叶片呈现轻微叶缘皱缩,块茎未出现畸形只存在芽眼变深症状,病毒侵染速度相对慢但病毒含量居中。PVA不同株系的致病性分析为其有效防治提供了有价值的理论依据。
Potato virus A(PVA)is one of the common viruses in potato production.Symptoms of plants infected with the virus are related to varieties,virus strains and environmental conditions.In order to study the pathogenicity of different PVA isolates to potato,the indicator plant’Rustica Tobacco’and the potato variety’Kexin 13’were inoculated with three PVA isolates with different genetic backgrounds.The qRT-PCR detection technology was used to identify virus infection and observe the difference of pathogenicity.All the isolates infected’Rustica Tobacco’and’Kexin 13’successfully.According to the leaf symptoms of’Rustica Tobacco’inoculated with the three isolates,it was inferred that the pathogenicity of the three PVA isolates was different.The virulence of isolate 2017-171 was the strongest in potato,which not only had the highest virus content in the plant,but also caused leaf edge shrinkage,mild mosaic,severe tuber crack and deep eye.Secondly,the isolate KB117 had stronger pathogenicity,faster virus infection speed,but lower virus content.The symptoms of infected plants were relatively obvious,with sharp and wavy leaf margins,deformed tubers and deep eyes.Isolate 2017-176 had the lowest pathogenicity.Only a few leaves showed slight leaf edge shrinkage and tubers were not deformed,only with deep eyes.Virus infection speed was relatively slow,but virus content was in the middle.The results of this experiment provide valuable theoretical basis for strain identification,prevention and control of PVA.
作者
张威
魏旭言
高艳玲
范国权
邱彩玲
孙旭红
武新娟
张俐俐
白艳菊
ZHANG Wei;WEI Xuyan;GAO Yanling;FAN Guoquan;QIU Cailing;SUN Xuhong;WU Xinjuan;ZHANG Lili;BAI Yanju(Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086,China;College of Agronomy and Life Sciences,Zhaotong University,Zhaotong,Yunnan 657000,China)
出处
《中国马铃薯》
2021年第6期568-574,共7页
Chinese Potato Journal
基金
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-09)
“农业科技创新跨越工程”专项(HNK2019CX07)。
