摘要
测定马铃薯M病毒(Potato virus M,PVM)寄主范围,并作PVM接种条件优化试验。结果表明,PVM仅侵染15种指示植物中的4种,各种植物症状表现不同。苋色黎(Chenopodium amaranticolor)和千日红(Gomphrena globosa L.)接种叶片上产生枯斑,可作为PVM枯斑寄主;36号烟(Nicotiana occidentalis"pi")系统发病,新生叶片产生皱缩、泡斑、叶边缘卷曲等症状,植株长势矮小,可作为PVM系统侵染鉴定寄主;PVM系统侵染番茄(Lycopersicon pimpinellifolium)速度快,但无症状,番茄可作为PVM繁殖寄主。PVM虽可侵染不同生育期寄主,以接种中上部叶位侵染效果最好,但在不同寄主不同叶位分布并不一致,番茄体内中部叶片病毒含量相对较高,在苋色黎上则分布较均匀。明确PVM最适培养温度为23~29℃。最高稀释度64倍时(OD405=0.201)仍可成功侵染苋色黎。为PVM纯毒源扩繁及制备该病毒抗血清提供基础数据。
In this study, the host range of potato virus M（PVM） was determined, and on the basis of this, PVM inoculation conditions were optimized. The results showed that PVM only infected four indicator plants among the 15 species tested, and the symptoms were different. On the inoculated leaves of Chenopodium amaranticolor and Gomphrena globosa L. expressed lesion, so they could be used as the lesion host of PVM. The Nicotiana occidentalis ＂pi＂ expressed systemic symptom, on the new leaves of which expressed crinkling, bubble spot, leaf curl and dwarf, so it could be used as the identification host of systemic infection. Lycopersicon pimpinellifolium could be systemically infected. The speed was fast, but did not show symptoms, so it could be used as the breeding host of PVM. Although PVM could infect the host plants of different growth stages, the leaves at mid-upper positions got the best infection result. However,the concentration of PVM was inconsistent between the leaves of different positions, with the virus content in leaves being relatively high in the middle part of the Lycopersicon pimpinellifolium, while the distribution in Chenopodium amaranticolor being even. The optimal culture temperature of PVM was 23-29 ℃. The highest dilution of 64 times（OD405=0.201） could still successfully infest Chenopodium amaranticolor. These results may provide the basic data for the preparation of the virus antiserum by the expansion of pure PVM.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2017年第1期1-6,共6页
Journal of Northeast Agricultural University
基金
现代农业产业技术体系专项资金资助项目(CARS-10-P14)
哈尔滨市科技创新人才专项资金项目(2015RQQYJ031)
