摘要
马铃薯Y病毒(Potato virus Y,PVY)是马铃薯生产中较常见的病毒之一,马铃薯病毒的检测是生产中保障马铃薯品质和产量的重要基础,收获后批量检测是进行种薯质量评价的主要依据之一。研究针对‘56-2’‘Ivory Russet’‘Clearwater Russet’‘龙薯14号’‘龙薯3号’‘龙薯15号’‘龙育201401-70’‘龙薯7号’‘克新23号’和‘克新28号’马铃薯品种(系)休眠块茎样品,采用TRIzol法提取马铃薯休眠块茎顶端和茎端芽眼组织的总RNA,经qRT-PCR和RT-PCR检测分析,比较块茎的顶端与茎端芽眼组织PVY含量分布的情况。结果表明,上述供试材料的休眠块茎茎端芽眼组织PVY浓度均高于顶端芽眼组织,能够精准的检测到病毒的存在;对‘Ivory Russet’品种的休眠块茎选取100个带病样品通过TRIzol法提取顶端与茎端的总RNA,采用4合1的方法合样后进行PVY RT-PCR检测,所有处理的顶端芽眼组织检测条带亮度低于茎端芽眼组织。可见,马铃薯休眠块茎茎端芽眼组织取样能够更加精准的检测出PVY。研究结果为马铃薯种薯收获后PVY的检测及合理取样提供了科学依据。
Potato virus Y(PVY)is one of the common viruses in potato production.Detection of potato virus is an important basis for safeguarding potato quality and yield in production.Batch detection after harvest is one of the main bases for seed potato quality evaluation.In this study,dormant tuber samples from the potato varieties(lines)of'56-2','Ivory Russet','Clearwater Russet','Longshu 14','Longshu 3','Longshu 15','Longyu 201401-70','Longshu 7','Kexin23'and'Kexin 28'were used to extract the total RNA in bud eyes tissues of apical end and stolon end by TRIzol method.The distribution of PVY content in bud eyes tissues of the apical end and stolon end was compared by qRTPCR and RT-PCR detection analysis.The PVY concentration in bud eyes tissues of the stolon end of the tested varieties(lines)was higher than that in the apical end,indicating that the presence of the virus could be accurately detected in stolon end.One hundred diseased tuber samples were selected from the dormant tubers of'Ivory Russet',and the total RNA in eye tissues of the apical end and stolon end was extracted by TRIzol method.The samples were combined by 4 in 1 method for PVY RT-PCR detection.The band brightness of apical end was lower than that of stolon end in all treatments,indicating that PVY could be detected more accurately using stolon end sample than apical end in dormant tubers.The results of this study provide a scientific basis for the reasonable sampling and detection of PVY after seed potato harvest.
作者
王新新
张威
刘尚武
尚慧
白艳菊
王彦杰
WANG Xinxin;ZHANG Wei;LIU Shangwu;SHANG Hui;BAI Yanju;WANG Yanjie(Agricultural College,Heilongjiang Bayi Agricultural University/Key Laboratory of Low-carbon Green Agriculture in Northeastern China,Ministry of Agriculture and Rural Affairs,Daqing,Heilongjiang 163319,China;Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086,China;Northeast Agricultural University,Harbin,Heilongjiang 150030,China;College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China)
出处
《中国马铃薯》
2023年第1期36-44,共9页
Chinese Potato Journal
基金
财政部和农业农村部国家现代农业产业技术体系(CARS-09-P16)
云南省科学技术厅重大专项(202102AE090018)。
