摘要
目前常用马铃薯病毒检测的方法,主要包括寄主生物学检测法、抗血清检测法、电子显微镜检测法、酶联免疫检测法和分子生物学检测法。重点讨论了分子生物学方法中的各种RT-PCR方法。同时对各种方法的优缺点进行了比较,并总结出将传统生物学检测技术、免疫学检测技术和分子生物学检测技术相结合必将成为检测植物病毒最经济有效的手段。
The common test methods for potato virus detection were reviewed, including host biology detection, antiserological detection assay, electron microscopy, enzyme-linked immunosorbent assay and molecular biological detection assay, with emphasis on the vadous RT-PCR methods of molecular biology methods. At the same time, the merit and demerit were compared with different methods, concluding that the combination of traditional biological detection, immunological detection and molecular biology detection technology would be the most cost-effective means for detection of plant viruses.
出处
《中国马铃薯》
2013年第4期239-242,共4页
Chinese Potato Journal
基金
定西师范高等专科学校校级科研项目(1316)
关键词
马铃薯病毒
检测方法
统计
分析
potato virus
detection method
statistic
analysis
