摘要
本文报道了１９８７～１９９０年对马铃薯主要病毒毒源试管封闭保存的研究结果．保毒植物为普通烟草、心叶烟、德伯尼烟、番茄、千日红，洋酸浆等．在无菌条件下，温度为２０～２５℃，２０００～３０００ｍ烛光照，取其０．５～１ｃｍ大茎尖消毒后扦插在试管培养基上培养，因保毒植物种类不同，其成活率为５７．６％～１００％，平均为８７．１％，进管２０～２６天生根发育正常．在试管内发育成株时，可进行单节切段更新繁殖，其生长发育比大茎尖初次进管时速度快，一般６～１２天生根发育正常，并保持试管毒源纯度和含毒量．
It is reported in this paper that the maintenance of main potato viruses source using in vitro culture technique was successful in 1987￣ 1990. The meristems of 0.5￣ 1cm were excised from Nicotiana tabacum, Nicotiana glutinosa, Nicotiana debneyi, Lycopersicon esculentum, Gomphrene globosa and Physalis floridana of the source plants and cultured on the medium in the test tubes after sterilization. The culture condition was 20￣25℃ and 2000￣ 3000 Lux. The survival rate was 57.6￣ 100%, depending on the different plants. The average rate was 87.1%. The excisments began rooting and growing normally in 20￣ 36 days.Multiplication by nodal cuttings could be started when the plantlets grown up .The cuttings began rooting and growing normally in 6￣ 12 days. And the purity and content of the viruses could be kept as well. Reference to this study have not been found as yet in China and overseas.
出处
《马铃薯杂志》
1996年第2期79-85,共7页
Chinese Potato Journal
关键词
马铃薯
病毒
保毒植物试管
试管保存技术
potato viruses, viruses source plants, maintenance in vitro technique.
