摘要
本研究从谷子基因组水平鉴定出33个SiGATA成员,命名为SiGATA1~SiGATA33。利用生物信息学方法对谷子SiGATA家族的系统发育、基因结构、染色体分布、保守基序、蛋白三级结构和顺式调控元件进行预测和分析。结果表明,33个SiGATA转录因子被划分为3组,不均匀的分布在8条染色体上,多数含有CX2CX18CX2C保守结构域;谷子GATA蛋白与单子叶植物水稻的同源性大于其他两种单子叶植物;蛋白三级结构分析表明,整体结构相似度存在差异,同组进化序列基因的结构相似度较高;顺式作用元件分析表明,SiGATAs启动子序列中存在调控生长发育、激素信号传导和逆境胁迫响应元件,其中SiGATA15、SiGATA22、SiGATA31在干旱、厌氧诱导及脱落酸响应等元件中表达量较高,其在谷子生长发育及逆境响应过程中可能发挥了重要生物学功能。这些逆境响应及生长发育相关转录因子的挖掘,将为作物育种提供一定理论参考。
The transcription factor GATA is widely distributed in eukaryotes and plays an important role in regulating plant flowering time,chlorophyll synthesis,carbon and nitrogen metabolism and senescence.In this study,33 SiGATA members were identified from the foxtail millet genome level and were named SiGATA1-SiGATA33.The bioinformatics method was used to predict and analyze the phylogeny,gene structure,chromosome distribution,conserved motifs,tertiary protein structure and cis-regulatory elements of the SiGATA family of foxtail millet.The results showed that 33 SiGATA transcription factors were divided into 3 groups,which were distributed unevenly on 8 chromosomes,and most of them contained CX2CX18CX2C conserved domains;the homology of foxtail millet GATA protein to monocot rice was greater than that of the other two monocot plants;the analysis of the tertiary structure of the protein showed that the overall structural similarity was different,and the structural similarity of the same set of evolutionary sequence genes was higher;the analysis of cis-acting elements showed that the SiGATAs promoter sequence contained elements that regulated growth and development,hormone signaling,and stress response.Among them,SiGATA15,SiGATA22,and SiGATA31 had higher expression levels in elements such as drought,anaerobic induction and ABA response,it may have an important biological function in the growth and development of foxtail millet and was in response to adversity.The mining of transcription factors related to these stress responses,the growth and the development will provide a certain theoretical reference for crop breeding.
作者
卢成达
李阳
孙迪
牛学谦
昌秦湘
LU Chengda;LI Yang;SUN Di;NIU Xueqian;CHANG Qinxiang(College of Agriculture,Shanxi Agricultural University,Taigu Shanxi 030801,China;Institute of Landscap Science,Taiyuan University,Taiyuan 030032,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2021年第5期645-654,共10页
Acta Agriculturae Boreali-occidentalis Sinica
基金
浙江省植物次生代谢与调控重点实验室开放基金(ZWCSDX-201901)
山西省重点研发计划(201803D221012-5)
山西省重点研发计划(201703D211002-4-2)
山西省深度贫困县科技精准扶贫专项(2019FP-12)。
