摘要
在高等植物中,SQUAMOSA Promoter Binding Protein-Box(SBP)普遍参与植物的生长、开花调控及细胞程序性死亡等过程。本研究对高粱(Sorghum bicolor L.)SBP转录因子家族进行鉴定并进行生物信息学分析,同时利用qRT-PCR对高粱SBP基因在PEG-6000模拟干旱胁迫下的表达进行分析,以验证其功能。结果表明,从高粱中共鉴定出19个SBP成员,除第8条染色体外,其他9条染色体上均分布有SBP基因;绝大部分SBP蛋白在亚细胞水平定位于细胞核,且均为亲水性蛋白质。系统发育分析结果表明,与水稻类似,这19个高粱SBP基因也可分为3个亚类,分别含有7、5、7个SBP基因;相比于拟南芥,高粱SBP与水稻、玉米的SBP关系更近。启动子顺式作用元件预测结果显示,高粱SBP可能参与了脱落酸、茉莉酸甲酯信号通路,部分成员可能与植物的低温响应、昼夜节律调控有关。qRT-PCR分析结果显示,高粱SBP基因的表达具有组织特异性,有17个基因受干旱胁迫诱导上调表达,且随胁迫时间的延长存在差异化表达模式。本研究结果可为后续研究高粱SBP基因的功能提供理论依据。
In the higher plants,SQUAMOSA promoter binding protein-box(SBP)is widely involved inplant growth,flowering regulation and programmed cell death.In this study,the sorghum SBP genes were identified,and then their bioinformatics analysis was conducted and their expression under PEG-6000 simulated drought stress was analyzed using qRT-PCR.The results showed that 19 members of sorghum SBP familywere identified.Except the 8th chromosome,the SBP genes were distributed on all the other 9 chromosomes.The SBP proteins were hydrophilic proteins,and most of them were located in nucleus at subcellular level.Similar to those of Oryza sativa,the sorghum SBP members also could be divided into three groups throughphylogenetic analysis,containing 7,5 and 7 members respectively;sorghum SBPs had closer relationshipswith those of Oryza sativa and Zea mays.The prediction results of cis-acting elements indicated that sorghum SBPs might participate in abscisic acid and methyl jasmonate signaling pathways,and some members might berelated to plant low temperature response and circadian rhythm regulation.qRT-PCR analysis results showedthat the expression of sorghum SBP genes were tissue-specific,and 17 genes were induced to up-regulate expression under simulated drought conditions and showed differentiated expression patterns with the extension ofstress time.These results could provide theoretical bases for subsequent research on SBP genes function.
作者
姜昱雯
陈满静
赵应
任艳
周棱波
沈佳奇
邵明波
Jiang Yuwen;Chen Manjing;Zhao Ying;Ren Yan;Zhou Lengbo;Shen Jiaqi;Shao Mingbo(Guizhou Institute of Upland Crops,Guiyang 550006,China;Renhuai Bureau of Agriculture and Rural Affairs,Renhuai 564500,China)
出处
《山东农业科学》
北大核心
2024年第3期1-10,共10页
Shandong Agricultural Sciences
基金
贵州省科技计划项目(黔科合基础-ZK[2023]一般169)
贵州省基层农技推广项目(仁怀基地示范[2301]01号)
贵州省科技成果应用及产业计划项目(黔科中引地[2022]4045)。
关键词
高粱
SBP基因家族
生物信息学分析
基因表达
干旱胁迫
Sorghum bicolor L.
SBP gene family
Bioinformatics analysis
Gene expression
Droughtstress
