摘要
WRKY转录因子是调控植物生长发育的重要转录因子,本研究利用高粱基因组数据库(v3.1.1),通过生物信息学分析鉴定出97个高粱WRKY基因家族成员,其CDS序列长度为423~4965 bp,编码氨基酸长度为141~1655 aa。染色体定位发现97个基因不均匀地分布于10条染色体上,其中3号染色体上数目最多,有23个WRKY基因,6号染色体最少,只有5个WRKY基因。根据WRKY保守结构域和锌指结构的特点对97个转录因子进行分类,可将它们分为Ⅰ、Ⅱ和Ⅲ三大类,分别有11个、70个和16个成员。另外对WRKY蛋白保守基序分析发现WRKY结构域有3个保守基序组成,各组成员的保守基序基本一致。部分蛋白的WRKY保守域和锌指结构具有多样性,分析发现部分高粱WRKY蛋白的WRKYGQK结构域中‘Q’突变为‘E’、‘S’和‘K’。系统进化关系显示高粱Ⅱ、Ⅲ类WRKY转录因子类聚在Ⅰ的下级分支上,表示Ⅱ和Ⅲ类可能是由Ⅰ类进化而来的。
WRKY transcription factor is an important transcription factor that regulates plant growth and development,In this study,97 sorghum WRKY gene family members were identified by bioinformatics analysis using sorghum genome Database(V3.1.1).The CDS sequence length was from 423 aa to 4965 bp and the encoded protein ammonia length was from 141 aa to 1655 aa.Chromosomal location analysis indicated that 97 genes were unevenly distributed on 10 chromosomes.The chromosome 3 has 23 genes which is most,while the chromosome6 only has 5 genes,which is fewest.According to the characteristics of WRKY domain and zinc finger structure,97 WRKYs can be divided into three groups:Ⅰ,ⅡandⅢ,with 11,70 and 16 members respectively.In addition,the conserved motif analysis of the protein revealed that WRKY domain has three conserved motifs,and the conserved motifs of the WRKY conserved domain and zinc finger structure of some proteins are diverse,and the WRKY conserved domain is mutated during evolution.Three mutation types were found that the’Q’mutated into’E’,’S’and’K’in the WRKYGQK conserved domain of WRKYs.The phylogenetic relationship showed that WRKY transcription factors of theⅡandⅢwere clustered on the sub-branch ofⅠ,which indicated thatⅡandⅢprobably evolved fromⅠ.
作者
赵兴奎
范昕琦
聂萌恩
王海燕
柳青山
段永红
Zhao Xingkui;Fan Xinqi;Nie Mengen;Wang Haiyan;Liu Qingshan;Duan Yonghong(College of Agriculture,Shanxi Agricultural University,Taigu,030801;Shanxi Key Laboratory of Sorghum Genetic and Gemplasm Innovation,Sorghum Istitute of Shanxi Academy of Agricutural Sciences,Jinzhong,03060)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第13期4170-4181,共12页
Molecular Plant Breeding
基金
山西省农业科学院博士研究基金项目(YBSJJ1607)
山西省农业科学院育种工程项目(17yzgc030)
山西省业科学院攻关项目(YYS1702)
山西省重点研发计划重点项目(201703D211010)
山西省自然科学基金项目(201701D120015)共同资助。
