摘要
GATA因子在植物中广泛参与了光响应调控、叶绿素合成以及碳、氮代谢等与作物产量息息相关的生物学过程。本研究基于蓖麻基因组信息,鉴定了19个蓖麻GATA家族成员,根据基因结构、蛋白结构和系统进化分析将其分为4个亚家族。理化性质分析发现GATA蛋白具有亲水性。基因和蛋白结构分析发现,蓖麻与拟南芥的GATA成员在结构上非常保守。利用高通量RNA-seq测序数据分析蓖麻GATA基因在各个组织中的表达情况,发现GATA基因主要在根和叶中表达,在种子中表达较低。进一步通过半定量RT-PCR技术检测GATA基因在黑暗下的表达情况,结果显示有三个GATA基因在黑暗处理后表达下调,预示着蓖麻GATA基因参与了光响应调控过程。这些信息为蓖麻GATA基因家族的功能分析和提高蓖麻产量提供了理论基础。
GATA proteins are considered to be broadly involved in yield-associated biological process, such as pho- toresponse, chlorophyll biosynthesis, and carbon and nitrogen metabolism. Based on castor bean genome database, a total of 19 GATA genes were identified and classified into 4 subfamilies according to gene structure, protein structure and their phylogenetic relationships. Results exhibited that GATA factors were hydrophilic proteins. Analysis of gene structure and protein structure revealed the conserved structural features of GATA factors between castor bean and Arabidopsis thaliana. The high-throughput RNA-seq data were used to define the expressional profiles of GATA genes among tissues. The results showed that most of the castor GATA genes preferentially expressed in leaf and root in con- trast to their expression in developing seeds. In particular, the expression of GATA genes responding to darkness treatment in leaves was detected using semi-quantitative RT-PCR. It was shown that expression of three genes was down-regulated under darkness treatment, which suggests a role for GATA genes of castor bean in light-mediated reg- ulation. These results provide important theoretical basis to the functions identification of castor GATA genes and in- crease castor yields.
出处
《植物分类与资源学报》
CAS
CSCD
北大核心
2015年第4期453-462,共10页
Plant Diversity
基金
国家自然科学基金(31401421)
