摘要
AP2亚组类转录因子是植物特有的一类转录因子,属于AP2/ERF基因家族,在植物生长发育、细胞分化以及响应生物和非生物胁迫过程中起着重要的作用。本研究通过生物信息学的方式共鉴定到16个水稻AP2基因,并对这些基因的理化性质、基因结构、进化途径、顺式调控元件、表达谱等方面进行分析。结果显示这些基因不均匀地分布在8条染色体上,根据同源性差异又可以分成3个亚组。每个亚组成员在基因结构以及保守基序的位置和数目上具有相似性,而且大部分OsAP2基因受到一个或者多个miRNA基因的调控。另一方面,这些基因在不同组织的表达水平有很大的差异,其中茎和花药中表达量相对较高,且部分OsAP2基因的表达量受到激素的诱导。这些结果为下一步研究水稻AP2基因的生物学功能打下坚实的基础。
AP2(APETALA2)-like transcription factors is a plant-specific transcription factor,belonging to AP2/EREBP(APETALA2/ethylene-responsive element binding proteins)gene family,which plays an important role in the growth and development,cell differentiation and responses to biotic and abiotic stresses in plant.The study identified 16 AP2 genes in the whole genome of rice through bioinformatics method.The physicochemical properties,gene structure,phylogenetic tree,cis-regulatory elements and expression profiles of these genes are analyzed.The results showed that OsAP2 genes were unequally distributed in 8 chromosomes and could be divided into three subgroups according to their homology.Gene structure and number of conserved motifs of each subgroup was similarity.In addition,most OsAP2 genes can be regulated by one or more miRNAs.On the other hand,the expression levels of OsAP2 genes are quite different,and highly expression was found in shoot and anther.Moreover,some OsAP2 genes expression was induced by hormone.These results provide a basis for further study of the biological function of OsAP2 genes.
作者
黄霞
柯笑楠
胡海超
郭文萍
蒋小玥
潘项健
Huang Xia;Ke Xiaonan;Hu Haichao;Guo Wenping;Jiang Xiaoyue;Pan Xiangjian(College of Agriculture and Food Sciences,Zhejiang A&F University,Hangzhou,311300)
出处
《分子植物育种》
CAS
北大核心
2021年第11期3489-3498,共10页
Molecular Plant Breeding
基金
学校科研发展基金项目(2019FR053)资助。
关键词
AP2转录因子
进化树
表达谱
顺式作用元件
MIRNA
AP2 transcription factors
Phylogenetic tree
Expression profiles
cis-regulatory elements
miRNA
