摘要
大薯属于薯蓣科薯蓣属,是热带和亚热带地区的一种重要的粮食作物,但其基础研究还较为薄弱。本研究基于公布的大薯转录谱序列和基因组序列,开展了对大薯ERF家族基因的挖掘和特征分析。共找到DaERF基因41个,其中14个DaERF有一个以上的外显子,其余的均为一个外显子。进一步的blastx分析发现30个DaERF基因具有保守的AP2结构域,依据AP2序列构建系统进化树,可将其分成5个亚家族。通过对AP2结构域的两个保守motif的位置和结合位点的分析,发现相同家族的ERF蛋白具有相似的结构特征。根据大薯转录谱分析DaERF在不同组织中的表达,发现一些DaERF的表达具有组织特异性,并且属于同一亚家族基因的表达具有相似性。我们对在块茎中具有高表达的DaERF-DaERF24基因,使用RT-qPCR验证其在不同组织的表达,结果与转录谱数据一致。本研究通过对大薯ERF转录因子家族特性的初步挖掘,为以后研究ERF家族全基因分布、ERF基因功能验证和薯块生长发育机理提供依据。
Great yam (Dioscorea alata), belongs to the genus Dioscorea (family Dioscoreaceae), is an important staple crop in tropical and subtropical regions. However, the researches for this crop is still weak. In this study, we used the published EST, transcriptome and genomic sequences of great yam to identify and characterize ERF gene family. A total of 41 DaERF genes were obtained, of which 14 DaERF genes have more than one exon and rest genes have one exon. Through blastx analysis of the 41 DaERF genes, we found that 30 DaERF genes have conserved AP2 domains. Phylogenesis analyses of the 30 DaERFs indicated that these genes could be divided into five subfamilies. Analysis of the location and binding sites of two motifs in DaREF genes demonstrated that DaERFs in a same subfamily tend to have same structural features and suggested that DaERFs in a same family might have similar functions. Analysis of expression profiles for these DaERFs showed that DaERFs in a same ERF family tend to have similar expression level and tissue specific patterns. DaERF24, which was highly expressed in tuber tissues based on transcriptome data, was further confinned its expression pattern by fluorescence quantitative PCR. In this study, the characteristics of ERF transcription factor family in great yam were preliminarily excavated to lay a foundation for the future study of the whole gene distribution of ERF family, the functional verification of ERF gene and the growth and development mechanism of tuber.
作者
张金兰
张博
黄东益
肖勇
吴文嫱
许云
黄小龙
夏薇
Zhang Jinlan;Zhang Bo;Huang Dongyi;Xiao Yong;Wu Wenqiang;Xu Yun;Huang Xiaolong;Xia Wei(Hainan University Institute of Tropical Agriculture and Forestry, Haikou, 570100;Coconut Research Institute, Wenchang, 571300)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第7期2086-2093,共8页
Molecular Plant Breeding
基金
国家自然科学基金地区科学基金项目(31660413
31760406)资助
