摘要
【目的】对桑树GATA转录因子的生物学信息特征进行鉴定与分析,探究桑树GATA家族基因的功能及基因进化,为后续桑树分子育种及良种选育的相关研究提供一定的理论基础。【方法】利用生物信息学方法对桑树GATA家族进行全基因组鉴定、理化分析、顺势作用元件及保守结构域等分析。【结果】桑树GATA家族成员的理化性质存在明显差异,但亚细胞定位预测显示其全部位于细胞核上;GATA蛋白三级结构整体存在一定差异,但同亚族的相似度较高,对植物某一些生理反应过程可能存在一定的协同调控功能;系统进化分析将桑树GATA成员分为4个亚家族,同一亚族上的成员具有保守基序及相似基因结构;表达模式分析表明,GATA基因在桑树不同组织(根、表皮、叶片、冬芽和花)中的表达模式存在显著差异,其中大部分在根部具有较高的表达,这可能与桑树根部发育和抗旱性密切相关;桑树GATA启动子顺式作用元件分析显示,MnGATAs可能参与生物/非生物胁迫下的转录调控及植物激素信号传导调控。【结论】MnGATAs在桑树全基因组范围内共鉴定23个,具有明显的保守性,在桑树各组织中的表达模式存在显著差异,可能调控桑树在胁迫刺激下的生理反应。本研究为探究桑树GATA家族基因的功能、基因进化、分子育种及良种选育等研究提供了一定的理论基础。
【Objective】The biological information characteristics of GATA transcription factors of mulberry were identified and analyzed,and the function and gene evolution of mulberry GATA family genes were explored,which provided a certain theoretical basis for subsequent research on mulberry molecular breeding and improved variety selection.【Method】The genome-wide identification,physicochemical analysis,homeogenic elements and conserved domains of the mulberry GATA family were analyzed by bioinformatics methods.【Result】The physical and chemical properties of the members of the mulberry GATA family were significantly different,but the subcellular localization prediction showed that they were all located in the nucleus;There were certain differences in the tertiary structure of GATA proteins as a whole,but the similarity of the same subfamily was high,and there may be a certain synergistic regulation function in some physiological response processes of plants;Phylogenetic analysis,the mulberry GATA members were divided into 4 subfamilies,and members in the same subfamily had similar conservation motif and gene structure;Expression pattern analysis showed that there were significant differences in the expression patterns of GATA genes in different tissues(roots,epidermal,leaves,winter buds and flowers)of mulberry,most of which had higher expression in roots,which may be closely related to root development and drought resistance of mulberry;Analysis of cis-acting elements in the GATA promoter of mulberry showed that MnGATAs may be involved in transcriptional regulation and plant hormone signaling regulation under biotic/abiotic stress.【Conclusion】A total of 23 MnGATAs were identified in the whole genome of mulberry,with obvious conservation,and the expression patterns in various tissues of mulberry were significantly different,which may regulate the physiological response of mulberry under stress.The research on gene evolution,molecular breeding and selection of elite varieties provides a certain theoretical b
作者
刘江
刘刚
曾益春
代洁
危玲
姚永权
佟万红
唐清霞
黄盖群
LIU Jiang;LIU Gang;ZENG Yi-chun;DAI Jie;WEI Ling;YAO Yong-quan;TONG Wan-hong;TANG Qing-xia;HUANG Gai-qun(Sericultural Research Institute,Sichuan Academy of Agricultural Sciences,Nanchong,Sichuan 637000,China)
出处
《西南农业学报》
CSCD
北大核心
2022年第12期2724-2731,共8页
Southwest China Journal of Agricultural Sciences
基金
四川省科技计划项目(21ZDYF2183)
四川省农业科学院“1+9”揭榜挂帅科技攻关(1+9KJGG004)
四川省现代农业学科建设推进工程项目(2021XKJS093)
2022年省财政自主创新专项项目(2022ZZCX085)
四川省科技计划项目(2022NZZJ0022)
国家现代农业产业技术体系四川蚕桑创新团队项目(SCCXTD-2022-17)
南充市应用技术研究与开发项目(16YFZJ0095)。
