摘要
目的观察人参多糖对脑出血模型大鼠的干预效果及对海马区细胞凋亡的影响。方法2019年5—7月于西安医学院第二附属医院神经内科实验室进行实验,选取健康雄性SD大鼠50只,随机选取10只为正常组,制作脑出血模型大鼠40只,随机数字表法分为模型组及人参多糖低剂量组、中剂量组、高剂量组各10只。正常组及模型组给予生理盐水灌胃,人参多糖低剂量组给予2 mg/kg人参多糖,中剂量组4 mg/kg,高剂量组8 mg/kg,均灌胃干预3周。5组大鼠均进行脑组织观察,比较各组大鼠脑含水量、神经缺损评分、学习记忆能力评分、肿瘤坏死因子(TNF-α)、丙二醇(MDA)、B淋巴细胞瘤-2(Bcl-2)、磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)通路蛋白表达及细胞凋亡率。结果与正常组比较,模型组出现脑出血组织细胞间隙增大,神经细胞固缩,胶质细胞增生等变化;与模型组比较,低剂量组、中剂量组、高剂量组均显著减轻。模型组脑含水量、脑神经缺损评分及学习记忆能力评分高于正常组,而人参多糖干预后上述改变均减轻,且呈剂量依赖性(F/P=41.499/0.001、12.442/0.001、34.601/0.001),模型组TNF-α、MDA含量高于正常组,而人参多糖干预后上述改变均减轻,且呈剂量依赖性(F/P=10.221/0.001、139.435/0.001);模型组24 h、48 h、72 h海马区细胞凋亡率均高于正常组,而人参多糖干预后上述改变均减轻,且呈剂量依赖性(F/P=17.806/0.001、14.978/0.001、22.363/0.001);模型组Bcl-2、Akt、PI3K低于正常组,而人参多糖干预后上述改变均减轻,且呈剂量依赖性(F/P=34.193/0.001、34.546/0.001、42.837/0.001)。结论人参多糖对脑出血模型大鼠作用显著,能够改善大鼠记忆能力,减少脑含水量、海马区细胞凋亡,降低脑出血后继发性损伤。
Objective To observe the intervention effect of ginseng polysaccharide on cerebral hemorrhage model rats and the effect on apoptosis of hippocampal cells.Methods The experiment was conducted in the neurology Laboratory of the Second Affiliated Hospital of Xi'an Medical College from May 2019 to July 2019.50 healthy male SD rats were selected,10 of them were randomly selected as a normal group,40 of them were made into cerebral hemorrhage model rats,all of which were successfully modeled,and randomly divided into model group,low dose group,middle dose group and high dose group.Normal group and model group were given normal saline,low dose group of ginseng polysaccharide was given 2 mg/kg,middle dose group was 4 mg/kg,high dose group was 8 mg/kg.The brain tissues of the five groups were observed,and the scores of nerve defect,brain water content,learning and memory ability were counted.The expression of tumor necrosis factor(TNF-α),propylene glycol(MDA),B lymphoma-2(Bcl-2),phosphatidylinositol 3-kinase/protein kinase B(P13K/Akt)pathway protein and apoptosis rate were detected.Results The brain water content,neurological deficit,and learning and memory ability of high dose group were lower than those of low dose group and middle dose group(F/P=41.499/0.001,F/P=12.442/0.001,F/P=34.601/0.001),while the content of TNF-αand MDA were lower than those of low dose group and middle dose group(F/P=10.221/0.001,F/P=139.435/0.001),and the apoptosis rate was lower than those of low dose group and middle dose group(F/P=17.806/0.001,F/P=14.978/0.001,F/P=22.363/0.001),Bcl-2,Akt and pl3k were higher than those of low dose group and middle dose group(F/P=34.193/0.001,F/P=34.546/0.001,F/P=42.837/0.001).Conclusion Ginseng polysaccharide has a significant effect on cerebral hemorrhage model rats,which can improve memory ability,reduce brain water content,apoptosis of hippocampal cells,and reduce secondary damage after cerebral hemorrhage.
作者
郑希院
翟洁敏
李会琪
赵龙斌
高章代
Zheng Xiyuan;Zhai Jiemin;Li Huiqi;Zhao Longbin;Gao Zhangdai(Department of Neurology,Second Affiliated Hospital of Xi'an Medical College,Xi’an 710038,China)
出处
《疑难病杂志》
CAS
2020年第4期394-398,共5页
Chinese Journal of Difficult and Complicated Cases
基金
陕西省重点研发计划项目(2017SF-282)。
关键词
人参多糖
脑出血
细胞凋亡
脑含水量
大鼠
Ginseng polysaccharide
Cerebral hemorrhage
Apoptosis
Brain water content
Rats
