摘要
目的观察脑泰通颗粒对缺血性脑卒中模型的作用，初步探究其治疗缺血性脑卒中的可能机制。方法对128只大鼠进行高脂血症造模，然后随机抽出30只作为假手术组，其余动物进行缺血性脑卒中造模，术后按随机数字表法随机分为安慰剂组、脑泰通颗粒组及阳性对照组，于造模后第2天开始灌胃给药，假手术组、安慰剂组给予生理盐水lml／100g·d^-1；脑泰通颗粒组给了脑泰通颗粒水溶液1ml／100g·d^-1，阳性对照组给予吡拉西坦片0．6g／100g·d^-1。各组均1次／d，持续4周，并分别于用药后7d、10d、14d、21d、28d观察大鼠的行为学改变、HE染色大鼠脑组织神经元变化及免疫组化方法检测脑组织中神经牛长导向因子Slit2和血管内皮生长因子（VEGF）的表达情况。结果造模后，动物均表现出病理征、偏瘫等局灶性神经症状，脑组织亦有明显软化灶。用药后实验组及阳性对照组动物改善良好，软化灶明显缩小。安慰剂组在7d、10d、14d、21d、28d的Longa评分分别为（2．92±0．20）分、（2．58±0．20）分、（2．25±0．27）分、（1．83±0．26）分、（1．42±0．20）分；脑泰通颗粒组评分分别为（1．92±0．20）分、（1．50±0．32）分、（1．25±0．27）分、（0．83±0．26）分、（0．50±0．00）分；阳性对照组评分分别为（2_33±0．41）分、（2．00±0．45）分、（1．75±0．27）分、（1．33±0．41）分、（0．92±0．38）分。与安慰剂组比较均降低（JP均〈0．01）。结论脑泰通颗粒可改善人鼠脑组织缺血缺氧状态，具有保护脑组织作用。
objective To explore the mechanism of Naotaitong granule on ischemic apoplexy byobserving the effect of Naotaitong granule on ischemic apoplexy model. Methods 128 rats were randomly divided into sham operation group, console group, experimental group and control group. Ischemic apoplexy model were established in all the animals. Different disposal was given to different group. In the 7 d, 10 d, 14 d, 21 d and 28 d after drug administration, immunohistochemistry were used to evaluate the expression of Slit2 and VEGF. In addition, behavior alteration and HE staining were applied to evaluate the variation of brain tissue neuron. Results All the animals revealed hemiplegia and cerebral tissue softened after successfully establishing apoplexy model. The morphology of experimental group and control group was improved after drug administration with softened focus obviously reduced. Longa grade of console group in the 7 d, 10 d, 14 d, 21 d and28dwas （2.92±0.20）, （2.58±0.20）, （2.25±0.27）, （1.83±0.26）and （1.42±0.20）respectively. Longa grade of Naotaitong group in the 7 d, 10 d, 14 d, 21 d and 28 d was （ 1.92 ± 0.20）, （ 1.50± 0.32）, （ 1.25 ±0.27 ） （0.83 ±0.26） and （0.50±0.00） respectively. Longa grade of control group in the 7 d, 10 d, 14 d, 21 d and 28 d was （2.33±0.41）, （2.00±0.45）, （1.75±0.27）, （1.33±0.41） and （0.92±0.38） respectively. Longa grade of both control and Naotaitong group had statistics significance compared with the console group （P〈0.01） . Conclusion Naotaitong granule may improve anoxemia in rats brain and protect brain tissue.
出处
《国际中医中药杂志》
2011年第9期791-794,共4页
International Journal of Traditional Chinese Medicine
基金
陕西省教育厅自然科学研究项目（项目编号：08JK277）：陕西省中医管理局自然科学研究项目（项目编号：jc69）
