摘要
利用DNA改善印迹孔穴对模板分子空间识别性能,建立了高选择性印迹材料的制备新方法,并用于痕量西马特罗的测定。在金电极表面自组装固定双链DNA,待西马特罗与DNA双链进行结合后,以西马特罗-DNA复合物为模板,电聚合制备西马特罗-DNA复合物的分子印迹聚合物膜。去除待测分子西马特罗,得到对西马特罗具有高特异性识别能力的分子印迹传感器。对传感器制备及工作条件进行了优化,利用紫外吸收光谱对西马特罗与DNA的结合方式进行了研究。结果表明,二者之间的相互作用模式为沟槽式结合。印迹膜以乙醇-乙酸(8∶1,V/V)为洗脱剂,洗脱时间为35 min,重吸附时间为45 min。以铁氰化物作为探针,其氧化电流与西马特罗浓度在1. 0×10^(-13)～1. 0×10^(-10)mol/L范围内呈线性关系,检出限为3. 2×10^(-14)mol/L(S/N=3)。将传感器成功应用于猪肉样品中西马特罗的检测,结果良好。
A novel strategy based on DNA induced conformation change was developed by rendering the imprinting cavity with refined structure in order to further improve the selectivity of molecular imprinting technology.The double-stranded DNA(dsDNA)was first self-assembled on the surface of the gold electrode,then the cimaterol molecule,taken as the representative target,was bound with the groove of dsDNA to form the complex template,together with which the molecular imprinting polymer(MIPs)was prepared by electropolymerization in the monomer solution.After elution of cimaterol,the obtained MIPs sensor had a remarkably high selectivity toward cimaterol.The combination process of cimetaerol and DNA was studied by UV-Vis and it was found to be the groove combination model.The optimum conditions of the system were optimized by electrochemical method.The results showed that when using ethanol-acetic acid(8∶1,V/V)as an eluent,with the elution time of 35 min and rebound time of 45 min,the sensor showed the best recognition ability toward cimaterol.This sensor exhibited a linear detection range of 1.0×10-13-1.0×10-10 mol/L,with the detection limit of 3.2×10-14 mol/L.The sensor was successfully applied to cimaterol detection in pork samples.
作者
张连明
张东友
曾英
李建平
ZHANG Lian-Ming;ZHANG Dong-You;ZENG Ying;LI Jian-Ping(Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541004,China;College of Materials and Chemistry&Chemical Engineering, Chengdu University of Technology,Chengdu 610059,China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2018年第11期1770-1777,共8页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金项目(No.21765006)
广西自然科学基金创新团队项目(No.2015GXNSFFA139005)
食品安全检测新技术广西高校重点实验室项目
桂林理工大学科研启动费项目(No.GUTQDJJ2016024)资助。
关键词
分子印迹
西马特罗
电化学传感器
DNA
选择性识别
Molecular imprinting
Cimaterol
Electrochemical sensor
DNA
Selective recognition
