摘要
A pre-treatment methodology for clenbuterol hydrochloride （CLEN） isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers （MIPs）. CLEN-imprinted pol- ymers were synthesized by the combined use of ally-β-cyclodextrin （ally-13-CD） and methacrylic acid （MAA）, allyl-β-CD and acrylonitrile （AN）, and allyl-β-CD and methyl methacrylate （MMA） as the binary functional monomers. MAA-linked allyl-β-CD MIPs （M-MAA） were characterized by Fourier transform-infrared （FT-IR） spectroscopy and a scanning electron microscope （SEM）. Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references： AN-linked allyl-β-CD MIPs （M-AN） and MMA-linked allyl-β-CD MIPs （M-MMA）. M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction （MISPE） micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation （RSD） ≤4.45%.
目的：测定猪肝脏中微量盐酸克仑特罗的残留量。创新点：开发一种新的瘦肉精检测方法。方法：用β-环糊精（ally—β—CD）和甲基丙烯酸（MAA）为功能单体制作分子印迹聚合物（MIP），以盐酸克伦特罗作为模板，用乙烯乙二醇二甲基丙烯酸酯（EGDMA）作为交联剂。联合使用ally-β-CD和MAA作为二元功能单体，能合成高质量的盐酸克伦特罗模板。
基金
Project supported by the Department of Science and Technology of Zhejiang Province(No.2013C02022-2/01),China
