摘要
试验采用紫外分光光度法研究典型兽药抗生素泰乐菌素(tylosin,TYL)和鲑鱼精DNA的相互作用,考察了TYL作用时间、浓度和溶液pH对鲑鱼精DNA结构的影响。结果表明,DNA紫外吸收值随着TYL浓度和作用时间的增加而减小;溶液pH=3.5~9.8时,DNA的紫外吸收值减小,且减小强度受TYL和DNA的带电情况影响。不同条件下,TYL对DNA结构产生的减色效应可能是由于TYL插入到DNA分子碱基对平面引起的。但pH=2.10时,TYL可能与DNA以沟槽式结合使得DNA结构表现出增色红移现象。由此可见,水环境中的兽药抗生素会影响介质中胞外DNA的稳定性。DNA结构的变化与抗生素作用时间、抗生素浓度和环境pH密切相关,其中pH的影响较为复杂。
In this paper,the ultraviolet spectroscopy is applied to study the correlation of tylosin（ a common veterinary antibiotic） and salmon sperm DNA and the effects of the interaction time,concentration of TYL and solution pH value on salmon sperm DNA structure are investigated. The results show the UV absorption value of DNA decreases with the increase of concentration and interaction time. Moreover,the reduced intensity of UV absorption value is affected by the charge of TYL and DNA at pH = 3. 5-9. 8. The hypochromicity at these conditions may be caused by the intercalation of TYL into base pair plane. However,TYL may bind DNA＇s groove at pH = 2. 10. As a result,the hyperchromicity and the red shift phenomenon occurs. Thus,veterinary antibiotic can influence the stability of extracellular DNA in water environment. The structure changes of extracellular DNA are related to the interaction time,concentration of TYL and solution pH,among which the effect of PH is more complex.
出处
《工业安全与环保》
北大核心
2017年第12期50-54,共5页
Industrial Safety and Environmental Protection
基金
国家自然科学基金(41501342)
广西自然科学基金(2016GXNSFBA380116)
广西大学生创新训练计划项目(201510595167)
