摘要
茶白星病是一种多年来严重为害高海拔茶园的叶部病害,为了突破该病害生物防控的技术瓶颈,明确不同茶白星病病情等级下叶际细菌群落具有重要意义。运用高通量测序方法比较分析3个不同发病等级的茶白星病叶片(BCK、B2和B4)中细菌群落组成,结果发现:在门水平上,3个病情等级下优势门均为Acidobacteria (酸杆菌门)、Bacteroidetes(拟杆菌门)、Proteobacteria(变形菌门)和Firmicutes(厚壁菌门),其中Proteobacteria(变形菌门)占总数的73.53%～90.25%。相对丰度多样性与茶白星病的发病病情等级呈正相关的为Acidobacteria(酸杆菌门)和Bacteroidetes(拟杆菌门)。在属水平上,随着病情等级升高,叶际细菌群落整体OTUs、差异OTUs及其多样性均上升。优势属Methylobacterium(甲醇杆菌属)与Hymenobacter(膜杆菌属)的相对丰度与病情等级呈正相关,而Ralstonia(雷氏菌属)与Stenotrophomonas(寡养单胞菌)则相反,表明茶白星病菌与叶际细菌存在协同作用,细菌群落在3个不同病情等级下存在差异,为进一步研究茶叶叶际微生态防控茶白星病提供了科学依据。
Tea white scab disease is a serious fungal disease in high altitude tea area for many years.Investigation of bacterial community and composition on tea in leaves with different severities of tea disease white scab disease will help improve its biological control.Results showed significant differences of bacterial community structures among 3 different severity levels.Based on the samples of three different severities(BCK,B2 and B4)were analyzed by high throughput DNA sequencing.At the phylum level,the dominants were Acidobacteria,Bacteroidetes,Proteobacteria and Firmicutes,and Proteobacteria in which accounts for 73.53%-90.25%.At the genus level,the relative abundance of Proteobacteria and Firmicutesunder was positively correlated with the severity of the disease.All of OTUs,differential OTUs and diversity of interfoliar bacterial community increased with the increase of disease grade.The relative abundance of Methylobacterium and Hymenobacter was positively correlated with the severity of the disease,while Ralstonia and Stenotrophomonas were opposite,indicating that they might have synergistic effect with the pathogen of tea white scab disease.These results provide a scientific reference for the further study of microecology on tea leaf and the prevention and control of tea white scab disease.
作者
周凌云
向芬
刘红艳
李维
周琳
银霞
曾泽萱
王振中
ZHOU Ling-yun;XIANG Feng;LIU Hong-yan;LI Wei;ZHOU Lin;YING Xia;ZENG Ze-xuan;WANG Zhen-zhong(Tea Research Institute of Hunan Academy of Agricultural Sciences,Changsha 410125,China;College of Agriculture,Agricultural University Of South China,Guangzhou 510642,China)
出处
《茶叶通讯》
北大核心
2019年第1期24-31,共8页
Journal of Tea Communication
基金
湖南省重点研发项目(2018NK2033)
国家重点研发计划(2016YED0200904)
广东省微生物信号与作物病害防控重点实验室开放基金(MSDC-02)
湖南省农业科学院创新项目(2017QN10)
关键词
茶叶
叶际细菌
茶白星病
细菌群落
多样性
功能预测
Tea
Phyllosphere bacteria
Tea white scab disease
Bacterial community
Diversity
Function prediction
