摘要
【背景】茶园根际土壤的细菌群落结构与茶园生境土壤营养循环密切相关,其组成及多样性可以作为健康茶园的一个生物指标。【方法】采用PCR-变性梯度凝胶电泳(PCR-DGGE)分子指纹图谱技术,检测安溪铁观音种植区不同海拔茶园根际土壤样本的细菌群落结构,利用Shannon-Wiener多样性指数分析其多样性,采用非加权组平均法进行聚类分析得到其分布特征,利用蒙特卡罗检验和冗余分析分别揭示影响细菌群落分布的环境因子及细菌群落分布和环境变量之间的关系。【结果】茶园根际土壤细菌的DGGE结果显示,检测到的14种主要细菌中有11种细菌是不可培养的,3种细菌是可培养的,分别属于根瘤菌属、中华根瘤菌属和苍白杆菌属。聚类分析得到,同一海拔梯度茶园根际土壤细菌群落结构相似。Shannon-Wiener多样性指数分析表明,400 m海拔处茶园根际土壤细菌群落多样性最高。蒙特卡罗检验分析得到环境因子协同作用对茶园根际土壤细菌群落结构贡献率为59.6%。冗余分析显示,茶园根际土壤细菌群落结构与海拔密切相关。【结论与意义】茶园根际土壤细菌群落结构分布与海拔梯度密切相关,考虑不同海拔高度土壤细菌群落对茶园营养循环的影响,在铁观音的健康栽培和管理过程中具有重要意义。
[ Background ] The structure and diversity of bacterial communities are closely related to the nutrient cycling in the rhizo sphere soil of tea plantations, which can be used as a biological basis for assessing the health status of tea plantations. [ Method] The bacterial communities in the rhizosphere soil of tea plantations located at different altitudes in Anxi County, Fujian Province, were detected and analyzed by the molecular fingerprinting technology of PCRdegeneration gradient gel electrophoresis （PCRDGGE）. The diversity of bacterial communities was measured using the Shannon diversity index. The distribution patterns of bacterial commu nities were clustered using the unweighted pairgroup method with arithmetic means, and the effects of environmental variables on the communities were tested using the method of Monte Carlo. The relationships between bacterial distributions and environmental variables were examined by redundancy analysis. [ Result] Among 14 species of bacteria obtained from the rhizosphere soil of tea plantations, 11 were unculturable species; the 3 culturable species were indentified as Rhizobium sp. , Sinorhizobium sp. and Ochrobactrum sp.. The cluster analysis of DGGE profile revealed similar patterns of the bacterial communities isolated from tea plan tations at the same altitude. The bacterial community with the highest Shannon diversity （H＇ = 3.27 ） appeared at the altitude of 400 m a. s. 1.. The synergistic effects of environmental variables explained 59.6% of the variation of bacterial communities in the rhizosphere soil. Redundancy analysis demonstrated that the bacterial communities in the rhizosphere soil were closely related to the altitudes of tea plantations. [ Conclusion and significance] The structure and diversity of bacterial communities are significantly associ ated with the altitudes, which may affect the efficiency of nutrient cycling and tea production, and that, the total phosphorus was the strongest environment variable factor influencing diversity of bacte
出处
《生物安全学报》
2013年第3期187-195,共9页
Journal of biosafety
基金
国际科技合作项目(2010DFB33030)
