摘要
为揭示烟草角斑病叶际微生物群落结构和多样性,采用高通量测序技术研究感角斑病与健康烟叶叶际细菌和真菌群落结构和多样性间的差异。结果表明,感病和健康烟叶叶际细菌主要分布于厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)和变形菌门(Proteobacteria);真菌主要分布于子囊菌门(Ascomycota)。在属水平上,感病烟叶叶际优势细菌属为假单胞菌属(Pseudomonas)、甲基杆菌属(Methylobacterium)、黄杆菌属(Xanthomonas)、寡养单胞菌属(Stenotrophomonas)、鞘氨醇单胞菌属(Sphingomonas)、泛菌属(Pantoea)和属水平未分类的根瘤科(unidentified_Rhizobiaceae);优势真菌属为小不整球壳属(Plectosphaerella)、亚格孢壳属(Didymella)、赤霉菌属(Gibberella)和链格孢属(Alternaria)。健康烟叶叶际优势细菌属为假单胞菌属、鞘氨醇单胞菌属、泛菌属和甲基杆菌属;优势真菌属为小不整球壳属、亚格孢壳属、枝孢霉属(Cladosporium)和Boeremia。健康烟叶叶际细菌、真菌群落丰富度和多样性指数均低于感病烟叶。功能预测显示,感病与健康烟叶叶际细菌代谢通路功能主要为代谢类、环境信息处理类和遗传信息处理类等。感病烟叶叶际真菌功能类群主要为植物病原菌、植物病原-未定义腐生菌、动物病原体-植物病原体-木材病原体-木腐生菌和内生植物病原菌;健康烟叶叶际真菌功能类群主要为植物病原菌、植物病原-未定义腐生菌、内生植物病原菌和未定义腐生菌。感病与健康烟叶叶际细菌和真菌群落结构、多样性、优势菌属及相对丰度均存在差异。
To understand the effects of phyllosphere microbial communities on tobacco leaves infected with angular leaf spot,the composition and diversity of phyllosphere microbial communities on diseased and healthy tobacco leaves were analyzed using high-throughput sequencing technology.The results showed that the phyllosphere bacteria of diseased and healthy tobacco leaves were mainly Firmicutes,Actinobacteria,and Proteobacteria,and the fungi were mainly Ascomycota at the phylum level.For diseased leaves,the dominant genera of bacteria were Pseudomonas,Methylobacterium,Xanthomonas,Stenotrophomonas,Sphingomonas,Pantoea,and unidentified_Rhizobiaceae,and those of fungi were Plectosphaerella,Didymella,Gibberella,and Alternaria.For healthy leaves,the dominant genera of bacteria were Pseudomonas,Sphingomonas,Pantoea,and Methylobacterium,and those of fungi were Plectosphaerella,Didymella,Cladosporium,and Boeremia.Richness and diversity indices of phyllosphere bacterial and fungal communities of diseased tobacco leaves were higher than those of healthy tobacco leaves.Functional predictions showed that the metabolic pathway functions of the bacteria in both diseased and healthy leaves were mainly metabolism,environmental information processing,and genetic information processing.The functional floras of fungi in diseased leaves were mainly plant pathogen,plant pathogen-undefined saprotroph,animal pathogen-endophyte-plant pathogen-wood saprotroph,and endophyte-plant pathogen.The functional floras of fungi in healthy leaves were mainly plant pathogen,plant pathogen-undefined saprotroph,endophyte-plant pathogen,and undefined saprotroph.Community composition,diversity,dominant genera and relative abundance of phyllosphere bacteria and fungi in diseased and healthy leaves differed to varying degrees.
作者
吴小军
汪汉成
孙美丽
蔡刘体
孟建玉
王丰
彭丽娟
WU Xiaojun;WANG Hancheng*;SUN Meili;CAI Liuti;MENG Jianyu;WANG Feng;PENG Lijuan(College of Tobacco,Guizhou University,Guiyang 550025,China;Guizhou Institute of Tobacco Science,Guiyang 550081,China;College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China)
出处
《烟草科技》
CAS
CSCD
北大核心
2023年第10期1-10,共10页
Tobacco Science & Technology
基金
国家自然科学基金项目“基于转录组学和表型组学的嘧菌酯抑制烟草立枯丝核菌菌核形成机理的研究”(31960550)
“烟草关键生育期叶际微生物群落结构及其时空分布格局和代谢功能研究”(32160522)
中国烟草总公司科技项目“基于叶际微生态的烟区多病害复合侵染机制研究”[110202101048(LS-08)]
贵州省人才项目“贵州省‘百层次’创新型人才”(GCC[2022]028-1)。
关键词
烟草角斑病
叶际微生物
群落结构
多样性
高通量测序
Tobacco angular leaf spot
Phyllosphere microorganism
Community composition
Diversity
High-throughput sequencing
