摘要
目的优化酶法提取土茯苓多糖的工艺。方法通过单因素试验法和响应面分析法,考察最佳酶的酶解时间、酶解p H、酶用量对提取土茯苓多糖的影响。结果木瓜蛋白酶提取多糖效率最高,其最佳工艺为酶用量0.6%,酶解p H为8,酶解时间150 min,在此条件下得到的实际多糖提取率为26.31%,与预测值25.71%的相对误差为2.33%。说明采用响应面分析法优化木瓜蛋白酶提取土茯苓多糖的方法可靠。结论此设计法用于酶法提取土茯苓多糖可行。
Objective To optimize the extraction technique of polysaccharide from Rhizoma Smilacis Glabrae by enzymatic method.Methods On the basis of single factor experimental and response surface methodology to investigate the influence of enzymatic time p H and dosage on extraction yield of polysaccharide.Results The optimum enzymatic was papain and its optimum conditions were as follows：enzyme concentration of 0.6%,enzymatic hydrolysis PH of 8,enzymatic hydrolysis time of 150 min,the enzymatic hydrolysis temperature of 50℃.Under these conditions above,the actual extraction ratio of polysaccharides from Rhizoma Smilacis Glabrae was26.31%,which was well matched with the predicted value（25.71%）.Conclusion This design method to extract polysaccharide from Rhizoma Smilacis Glabrae by enzymatic is feasible.
作者
郑朝阳
肖兰华
覃军
ZHENG Chaoyang;XIAO Lanhua;Q(The Second Clinical College, Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510006, China)
出处
《中国中医药现代远程教育》
2018年第6期99-102,共4页
Chinese Medicine Modern Distance Education of China
基金
广东省科技计划项目【No.2015B020211013
No.2016A040403104】
关键词
土茯苓
多糖
木瓜蛋白酶
响应面法
提取工艺
中药化学
Rhizoma Smilacis Glabrae
Polysaccharide
Papain
response surface method
extracting methods
chemistry of Chinese materia medica
